GATA-1 binding to the αV promoter negatively regulates expression of the integrin αV subunit in human leukemic K562 cells.

• Authors: Małgorzata Czyż , Marta Stasiak , Joanna Boncela , Czesław S Cierniewski
• Languages of publication: EN

Abstracts

EN

Recently we observed that the transcription factors Sp1 and Sp3 bind to the CTCCTCCTC sequence located between positions -194 and -172 of the αV promoter region and are directly involved in the regulation of transcriptional activity of the αV gene in human umbilical vascular endothelial cells (HUVECs) (Czyz & Cierniewski, 1999, Eur. J. Biochem. 265, 638). In this report we provide evidence that the GATA-1 factor regulates αV expression during differentiation of pluripotent K562 cells induced either by phorbol 12-myristate 13-acetate (PMA) or butyric acid (BA) through interaction with the GATA element in the αV gene promoter. DNase I footprinting analysis revealed that region -413 to -408, covering the GATA binding site, was protected by nuclear extract from K562 cells. There was no protection of this region by HUVEC nuclear extract. Electrophoretic mobility shift assay (EMSA) analysis of nuclear extract of K562 cells treated with BA revealed an increase in GATA binding activity, which was associated with reduced αV mRNA and αV protein on the cell surface. Stimulation of K562 cells with PMA resulted in opposite effects: lower expression of GATA-1 was associated with increased levels of αV. We conclude that the GATA-1 transcription factor specifically binds to the GATA element in the αV gene promoter and negatively regulates αV gene expression.

Keywords

EN

αvβreceptors; transcription regulation; integrins; gene expression

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2002
• Volume: 49
• Issue: 1
• Pages: 19-28

Dates

published

2002

received

2001-09-17

revised

2001-11-27

accepted

2002-01-15

Contributors

author

Małgorzata Czyż

• Department of Medicinal Chemistry, Institute of Physiology and Biochemistry, Medical University of Łódź, Łódź, Poland

author

Marta Stasiak

• Department of Biophysics, Institute of Physiology and Biochemistry, Medical University of Łódź, Łódź, Poland

author

Joanna Boncela

• Department of Biophysics, Institute of Physiology and Biochemistry, Medical University of Łódź, Łódź, Poland

author

Czesław S Cierniewski

• Center of Microbiology and Virology, Polish Academy of Sciences, Łódź, Poland

References

• 1. Dubant, J.L., Rocher, S., Chen, W.-T., Yamada, K.M. & Thiery, J.P. (1986) Cell adhesion and migration in the early vertebrate embryo: Location and possible role of putative fibronectin receptor complex. J. Cell Biol. 102, 160-173.
• 2. Chen, W.T., Chen, J.M. & Mueller, S.C. (1986) Coupled expression and colocalization of 140K cell adhesion molecules, fibronectin, and laminin during morphogenesis and cytodifferentiation of chick lung cells. J. Cell Biol. 103, 1073-1090.
• 3. Vol, T., Fessler, L.I. & Fessler, J.H. (1990) A role for integrin in the formation of sarcomeric cytoarchitecture. Cell 63, 525-536.
• 4. Zutter, M.M., Fong, A.M., Krigman, H.R. & Santoro, S.A. (1992) Differential regulation of the α2 β1 and αIIb β3 integrin genes during megakaryocytic differentiation of pluripotential K562 cells. J. Biol. Chem. 267, 20233-20238.
• 5. Kitazawa, S., Ross, F.P., McHugh, K. & Teitelbaum, S.L. (1995) Interleukin-5 induces expression of the integrin αV β3 via transactivation of the β3 gene. J. Biol. Chem. 270, 4115-4120.
• 6. Zambruno, G., Marchisio, P. C., Marconi, A., Vaschieri, C., Melchiori, A., Giannetti, A. & De Luca, M. (1995) Transforming growth factor- β1 modulates β1 and β5 integrin receptors and induces the de novo expression of the αV β6 heterodimer in normal human keratinocytes: Implications for wound healing. J. Cell Biol. 129, 853-865.
• 7. Enenstein, J., Waleh, N.S. & Kramer, R.H. (1992) Basic FGF and TGF-beta differentially modulate integrin expression of human microvascular endothelial cells. Exp. Cell Res. 203, 499-503.
• 8. Hynes, R.O. (1992) Integrins: Versatility, modulation, and signalling in cell adhesion. Cell 69, 11-25.
• 9. Bates, R., Rankin, L., Lucas, S., Scott, J., Krissansen, G. & Burns, G. (1991) Individual embryonic fibroblasts express multiple β chains in association with the αV integrin subunit. J. Biol. Chem. 266, 18593-18599.
• 10. Defilippi, P., Truffa, G., Stefanuto, G., Altruda, F., Silenge, L. & Tarone, G. (1991) Tumor necrosis factor α and interferon γ modulate the expression of the vitronectin receptor (integrin β3) in human endothelial cells. J. Biol. Chem. 266, 7638-7645.
• 11. Kim, L.T. & Yamada, K.M. (1997) The regulation of expression of integrin receptors. Proc. Soc. Exp. Biol. Med. 214, 123-131.
• 12. Medhora, M.M., Teitelbaum, S., Chappel, J., Alvarez, J., Mimura, H., Ross, F.P. & Hruska, K. (1993) 1α,25-dihydroxyvitamin D3 up-regulates expression of the osteoclast integrin LPH v beta 3. J. Biol. Chem. 268, 1456-1461.
• 13. Donahue, J.P., Sugg, N. & Hawiger, J. (1994) The integrin αV gene: Identification and characterisation of the promoter region. Biochim. Biophys. Acta 1219, 228-232.
• 14. Vila-Garcia, M., Li, L., Riely, G. & Bray, P.F. (1994) Isolation and characterisation of a TATA-less promoter for the human β3 integrin gene. Blood 83, 668-676.
• 15. Czyz, M. & Cierniewski, C.S. (1999) Selective Sp1 and Sp3 binding is crucial for activity of the integrin αV promoter in cultured endothelial cells. Eur. J. Biochem. 265, 638-644.
• 16. Kim, L.T., Wu, J. & Turnage, R.H. (1998) Integrin αV promoter activity in keratinocytes. J. Surg. Res. 76, 185-191.
• 17. Dai, W. & Murphy, M.J., Jr. (1993) Downregulation of GATA-1 expression during phorbol myristate acetate-induced megakaryocyte differentiation of human erythroleukemia cells. Blood 81, 1214-1221.
• 18. Chenais, B., Molle, I., Trentesaux, C. & Jeannesson, P. (1997) Time-course of butyric acid-induced differentiation in human K562 leukemic cell line: Rapid increase in γ-globin, porphobilinogen deaminase and NF-E2 mRNA levels. Leukemia 11, 1575-1579.
• 19. Chenais, B. (1998) Requirement of GATA-1 and p45 NF-E2 expression in butyric acid-induced erythroid differentiation. Biochem. Biophys. Res. Commun. 253, 883-886.
• 20. Shimamoto, T., Ohyashiki, J.H., Ohyashiki, K. & Toyama, K. (1995) The expression pattern of transcription factors (GATA, SCL) and biological characteristics in various leukemia cells. Rinsho-Ketsueki. 36, 547-551.
• 21. Dignam, J.D., Martin, P.L. & Roeder, R.G. (1983) Eukaryotic gene transcription with purified factors. Methods Enzymol. 101, 582-598.
• 22. Moll, T., Czyz, M., Holzmuller, H., Hofer- Warbinek, R., Wagner, E., Winkler, H., Bach, F.H. & Hofer, E. (1995) Regulation of the tissue factor promoter in endothelial cells. J. Biol. Chem. 270, 3849-3857.
• 23. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of micrograms quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
• 24. Kambe, M., Miyamoto, Y. & Hayashi, M. (1998) Characterization of the integrin αV gene promoter in mice. Biochim. Biophys. Acta 1395, 209-219.
• 25. Feng, X., Teitelbaum, S.L., Quiroz, M.E., Cheng, S.-L., Lai, C.-F., Avioli, L.V. & Ross, F.P. (2000) Sp1/Sp3 and PU.1 differentially regulate β5 integrin gene expression in macrophages and osteoblasts. J. Biol. Chem. 275, 8331-8340.
• 26. Noti, J.D., Johnson, A.K. & Dillon, J.D. (2000) Structural and functional characterization of the leukocyte integrin gene CD11d. J. Biol. Chem. 275, 8959-8969.
• 27. Li, J., Noguchi, C.T., Miller, W., Hardison, R. & Schechter, A.N. (1998) Multiple regulatory elements in the 5'-flanking sequence of the human ε-globin gene. J. Biol. Chem. 273, 10202-10209.
• 28. Amrolia, P.J., Cunningham, J.M., Ney, P., Nienhuis, A.W. & Jane, S.M. (1995) Identification of two novel regulatory elements within the 5'-untranslated region of the human A γ -globin gene. J. Biol. Chem. 270, 12892-12898.
• 29. Chin, K., Oda, N., Shen, K. & Noguchi, C.T. (1995) Regulation of transcription of the human erythropoietin receptor gene by proteins binding to GATA-1 and Sp1 motifs. Nucleic Acids Res. 23, 3041-3049.
• 30. Imagawa, S., Yamamoto, M. & Miura, Y. (1997) Negative regulation of the erythropoietin gene expression by the GATA transcription factors. Blood 89, 1430-1439.