Cytotoxic effect of nitric oxide on human hematological malignant cells.

• Authors: Michihiro Tsumori , Junko Tanaka , Kunio Koshimura , Mikiko Kawaguchi , Yoshio Murakami , Yuzuru Kato
• Languages of publication: EN

Abstracts

EN

We investigated the cytotoxic effect of nitric oxide (NO) on primary culture of human hematological malignant cells. Sodium nitroprusside (SNP), an NO donor, had cytotoxic effects on the cells of some patients with malignant lymphoma (ML), acute myelocytic leukemia (AML) or chronic myelomonocytic leukemia (CMMoL), but not with multiple myeloma. Cultured cells from the ML patient remained sensitive to SNP after the cells became resistant to anti-cancer drugs. In contrast, the cells from the patients with AML and CMMoL became resistant to SNP while anti-cancer drugs remained effective. In samples of the cells of the patients with ML and AML, the number of CD3 positive lymphoma cell was decreased by SNP and the number of CD33 negative cells and normal B lymphocytes (CD19 positive cells) were increased. In the cells of the patient with ML, apoptosis was induced by SNP. SNP had no effect on lymphocytes of healthy volunteers. These results suggest that SNP had an anti-tumor effect on human hematological malignant cells.

Keywords

EN

lymphoma; myeloma; leukemia; nitric oxide

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2002
• Volume: 49
• Issue: 1
• Pages: 139-144

Dates

published

2002

received

2001-09-10

revised

2002-01-29

accepted

2002-02-12

Contributors

author

Michihiro Tsumori

• First Division, Department of Medicine, Shimane Medical University, 89-1 Enya-cho, Izumo, 693-8501, Japan

author

Junko Tanaka

• First Division, Department of Medicine, Shimane Medical University, 89-1 Enya-cho, Izumo, 693-8501, Japan

author

Kunio Koshimura

• First Division, Department of Medicine, Shimane Medical University, 89-1 Enya-cho, Izumo, 693-8501, Japan

author

Mikiko Kawaguchi

• First Division, Department of Medicine, Shimane Medical University, 89-1 Enya-cho, Izumo, 693-8501, Japan

author

Yoshio Murakami

• First Division, Department of Medicine, Shimane Medical University, 89-1 Enya-cho, Izumo, 693-8501, Japan

author

Yuzuru Kato

• First Division, Department of Medicine, Shimane Medical University, 89-1 Enya-cho, Izumo, 693-8501, Japan

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