Detection and sequencing of new cyclic peptides from linseed by electrospray ionization mass spectrometry.

• Authors: Piotr Stefanowicz
• Languages of publication: EN

Abstracts

EN

Extract of Linum usitatissimum seeds was analyzed by ESI-MS and ESI-MS/MS. The analysis confirms the presence of previously reported cyclolinopeptides: CLA (c(Pro-Pro-Phe-Phe-Leu-Ile-Ile-Leu-Val) and CLB (c(Pro-Pro-Phe-Phe-Val-Ile-Met-Ile-Leu)). Cyclolinopeptides CLD and CLE, which contain methionine oxide, were detected in small quantities only. These peptides likely result from the oxidation of their precursors, not reported previously: CLD' (c(Pro-Phe-Phe-Trp-Ile-Met-Leu-Leu)) and CLE' (c(Pro-Leu-Phe-Ile-Met-Leu-Val-Phe)), present at higher concentrations in unoxidized extract. Two new cyclic octapeptides: CLF (c(Pro-Phe-Phe-Trp-Val-Met-Leu-Met)) and CLG (c(Pro-Phe-Phe-Trp-Ile-Met-Leu-Met)) were detected and their sequences were proposed on the basis of CID experiments and similarity with those of CLD'.

Keywords

EN

cyclolinopeptides; mass spectrometry

• Journal: Acta Biochimica Polonica
• Year: 2001
• Volume: 48
• Issue: 4
• Pages: 1125-1129

Dates

published

2001

received

2001-09-19

accepted

2001-11-26

Contributors

author

Piotr Stefanowicz

• Faculty of Chemistry, University of Wrocław, Wrocław, Poland

References

• 1. Wieczorek, Z., Bengtson, B., Trojnar, J. & Siemion, I.Z. (1991) Peptide Res 4, 275-283 .
• 2. Gaymes, T.J., Cebrat, M., Siemion, I.Z. & Kay, J.E. (1997) FEBS Lett 418, 224-227.
• 3. Morita, H., Shishido, A., Matsumoto, T., Itokawa, H. & Takeya, K. (1999) Tetrahedron 55, 967-976 .
• 4. Kessler, H., Haupt, M., Frimmer, K. & Zigler, K. (1986) Int. J. Peptide Protein Res. 29, 621-628 .
• 5. Bell, A., McSteen, P.M., Cebrat, M., Picur, B. & Siemion, I.Z. (2000) Acta Pol. Pharm 57, (Suppl. ) 134-136.
• 6. Kaufmann, H.P. & Tobschirbel, A. (1959) Chem. Ber 92, 2805-2809 .
• 7. Weygand, F. (1968) Z. Anal. Chem 243, 2 .
• 8. Eckart, K. (1994) Mass Spectrom. Rev 13, 23-55 .
• 9. Nielsen, H.K., Loliger., J. & Hurrell, R.F. (1985) Br. J. Nutr 53, 61-73.