Enhanced microdialysis of neuropeptides.

• Authors: Andreas Pettersson , Karin Markides , Jonas Bergquist
• Languages of publication: EN

Abstracts

EN

An enhanced microdialysis method for neuropeptides is described and some preliminary results of this novel approach are presented. The enhancement is achieved by adding a vehicle (solid support) to the perfusion fluid in order to increase the diffusion coefficient across the membrane and efficiently transport the analytes towards the detector. The microdialysis samples are desalted and then analyzed on an electrospray ionization orthogonal time-of-flight mass spectrometer. The preliminary results show major increase in signal when comparing this new approach of microdialysis with ordinary microdialysis.

Keywords

EN

neuropeptides; electrospray ionization orthogonal time-of-flight mass spectrometry; enhanced microdialysis

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2001
• Volume: 48
• Issue: 4
• Pages: 1117-1119

Dates

published

2001

received

2001-09-19

accepted

2001-11-10

Contributors

author

Andreas Pettersson

• Department of Analytical Chemistry, Uppsala University, P.O. Box 531, SE-75121 Uppsala, Sweden

author

Karin Markides

• Department of Analytical Chemistry, Uppsala University, P.O. Box 531, SE-75121 Uppsala, Sweden

author

Jonas Bergquist

• Department of Analytical Chemistry, Uppsala University, P.O. Box 531, SE-75121 Uppsala, Sweden

References

• 1. Ungerstedt, U. (1991) J. Intern. Med. 230, 365-373.
• 2. Davies, M.I. (1999) Anal. Chim. Acta 379, 227-249.