Direct in vivo transfer of plasmid DNA into murine tumors: Effects of endotoxin presence and transgene localization.

• Authors: Magdalena Budryk , Tomasz Cichoń , Stanisław Szala
• Languages of publication: EN

Abstracts

EN

The purpose of this study was to investigate the effect of endotoxin presence in plasmid DNA preparations on the efficiency of transfection achieved in vivo with B16(F10) and Renca tumors and to determine transgene localization. Our data show that endotoxin markedly decreases the efficiency of transfection. Furthermore, the transgene transferred in vivo can be found in both neoplastic and normal (most likely myofibroblast) cells lying in proximity of the administration site.

Keywords

EN

DNA transfer in vivo; endotoxin; myofibroblasts; naked DNA

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2001
• Volume: 48
• Issue: 3
• Pages: 795-800

Dates

published

2001

received

2001-04-18

revised

2001-07-04

accepted

2001-07-25

Contributors

author

Magdalena Budryk

• Department of Molecular Biology, Center of Oncology - Maria Skłodowska-Curie Memorial Institute, Gliwice, Poland

author

Tomasz Cichoń

• Department of Molecular Biology, Center of Oncology - Maria Skłodowska-Curie Memorial Institute, Gliwice, Poland

author

Stanisław Szala

• Department of Molecular Biology, Center of Oncology - Maria Skłodowska-Curie Memorial Institute, Gliwice, Poland

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