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2001 | 48 | 3 | 795-800
Article title

Direct in vivo transfer of plasmid DNA into murine tumors: Effects of endotoxin presence and transgene localization.

Content
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EN
Abstracts
EN
The purpose of this study was to investigate the effect of endotoxin presence in plasmid DNA preparations on the efficiency of transfection achieved in vivo with B16(F10) and Renca tumors and to determine transgene localization. Our data show that endotoxin markedly decreases the efficiency of transfection. Furthermore, the transgene transferred in vivo can be found in both neoplastic and normal (most likely myofibroblast) cells lying in proximity of the administration site.
Publisher

Year
Volume
48
Issue
3
Pages
795-800
Physical description
Dates
published
2001
received
2001-04-18
revised
2001-07-04
accepted
2001-07-25
Contributors
  • Department of Molecular Biology, Center of Oncology - Maria Skłodowska-Curie Memorial Institute, Gliwice, Poland
  • Department of Molecular Biology, Center of Oncology - Maria Skłodowska-Curie Memorial Institute, Gliwice, Poland
  • Department of Molecular Biology, Center of Oncology - Maria Skłodowska-Curie Memorial Institute, Gliwice, Poland
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv48i3p795kz
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