Antioxidative defense to lead stress in subcellular compartments of pea root cells.

• Authors: Arleta Małecka , Wiesława Jarmuszkiewicz , Barbara Tomaszewska
• Languages of publication: EN

Abstracts

EN

Lead, similar to other heavy metals and abiotic factors, causes many unfavorable changes at the subcellular and molecular levels in plant cells. An increased level of superoxide anion in Pisum sativum root cells treated with 1 mM Pb(NO3)2 evidenced oxidative stress conditions. We found increased activities of enzymatic components of the antioxidative system (catalase and superoxide dismutase) in the cytosol, mitochondrial and peroxisomal fractions isolated from root cells of Pisum sativum grown in modified Hoagland medium in the presence of lead ions (0.5 or 1 mM). Two isoenzyme forms of superoxide dismutase (Cu,Zn-SOD and Mn-SOD) found in different subcellular compartments of pea roots were more active in Pb-treated plants than in control. Increased amount of alternative oxidase accompanied by an increased activity of this enzyme was found in mitochondria isolated from lead-treated roots. These results show that plants storing excessive amounts of lead in roots defend themselves against the harmful oxidative stress caused by this heavy metal.

Keywords

EN

lead; superoxide dismutase; Pisum sativum; catalase; peroxisomes; superoxide anion; alternative oxidase; mitochondria; oxidative stress

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2001
• Volume: 48
• Issue: 3
• Pages: 687-698

Dates

published

2001

received

2001-01-22

revised

2001-05-9

accepted

2001-08-30

Contributors

author

Arleta Małecka

• Department of Biochemistry, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznań, Poland

author

Wiesława Jarmuszkiewicz

• Department of Bioenergetics, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznań, Poland

author

Barbara Tomaszewska

• Department of Biochemistry, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznań, Poland

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