Isoprenoid biosynthesis via 1-deoxy-D-xylulose 5-phosphate/2-C- methyl-D-erythritol 4-phosphate (DOXP/MEP) pathway.

• Authors: Małgorzata Wanke , Karolina Skorupinska-Tudek , Ewa Swiezewska
• Languages of publication: EN

Abstracts

EN

Higher plants, several algae, bacteria, some strains of Streptomyces and possibly malaria parasite Plasmodium falciparum contain the novel, plastidic DOXP/MEP pathway for isoprenoid biosynthesis. This pathway, alternative with respect to the classical mevalonate pathway, starts with condensation of pyruvate and glyceraldehyde-3-phosphate which yields 1-deoxy-D-xylulose 5-phosphate (DOXP); the latter product can be converted to isopentenyl diphosphate (IPP) and eventually to isoprenoids or thiamine and pyridoxal. Subsequent reactions of this pathway involve transformation of DOXP to 2-C-methyl-D-erythritol 4-phosphate (MEP) which after condensation with CTP forms 4-diphosphocytidyl-2-C-methyl-D-erythritol (CDP-ME). Then CDP-ME is phosphorylated to 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) and to 2-C-methyl-D-erythritol-2,4-cyclodiphosphate (ME-2,4cPP) which is the last known intermediate of the DOXP/MEP pathway. Formation of IPP and dimethylallyl diphosphate (DMAPP) from ME-2,4cPP still requires clarification. This novel pathway appears to be involved in biosynthesis of carotenoids, phytol (side chain of chlorophylls), isoprene, mono-, di-, tetraterpenes and plastoquinone whereas the mevalonate pathway is responsible for formation of sterols, sesquiterpenes and triterpenes. Several isoprenoids were found to be of mixed origin suggesting that some exchange and/or cooperation exists between these two pathways of different biosynthetic origin. Contradictory results described below could indicate that these two pathways are operating under different physiological conditions of the cell and are dependent on the developmental state of plastids.

Keywords

EN

isoprenoids; isopentenyl diphosphate; DOXP/MEP pathway; MVA pathway; pyruvate; glyceraldehyde phosphate

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2001
• Volume: 48
• Issue: 3
• Pages: 663-672

Dates

published

2001

received

2001-07-10

revised

2001-08-22

accepted

2001-09-4

Contributors

author

Małgorzata Wanke

• Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland

author

Karolina Skorupinska-Tudek

• Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland

author

Ewa Swiezewska

• Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland

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