An introduction to DNA chips: principles, technology, applications and analysis.

• Authors: Magdalena Gabig , Grzegorz Węgrzyn
• Languages of publication: EN

Abstracts

EN

This review describes the recently developed GeneChip technology that provides efficient access to genetic information using miniaturised, high-density arrays of DNA or oligonucleotide probes. Such microarrays are powerful tools to study the molecular basis of interactions on a scale that would be impossible using conventional analysis. The recent development of the microarray technology has greatly accelerated the investigation of gene regulation. Arrays are mostly used to identify which genes are turned on or off in a cell or tissue, and also to evaluate the extent of a gene's expression under various conditions. Indeed, this technology has been successfully applied to investigate simultaneous expression of many thousands of genes and to the detection of mutations or polymorphisms, as well as for their mapping and sequencing.

Keywords

EN

gene expression; DNA array; oligonucleotide array; hybridisation

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2001
• Volume: 48
• Issue: 3
• Pages: 615-622

Dates

published

2001

received

2001-04-3

revised

2001-09-4

accepted

2001-09-5

Contributors

author

Magdalena Gabig

• Department of Biotechnology, Royal Institute of Technology, Stockholm, Sweden

author

Grzegorz Węgrzyn

• Department of Molecular Biology, University of Gdańsk, Gdańsk, Poland

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