Repair of γ-ray-induced base damage in L5178Y sublines is damage type-dependent and unrelated to radiation sensitivity.

• Authors: Marcin Kruszewski , Tomasz H Zastawny , Irena Szumiel
• Languages of publication: EN

Abstracts

EN

The L5178Y (LY) murine lymphoma sublines LY-R and LY-S are differentially sensitive to ionizing radiation. The high radiation sensitivity of LY-S cells is related to impaired rejoining of DNA double strand breaks. We found previously that the γ-ray-induced base damage is higher in the more radiosensitive LY-S subline. Here, we examine the role of the repair of ionizing radiation induced base damage in relation to the radiosensitivity difference of these sublines. We used the GS/MS technique to estimate the repair rates of six types of base damage in γ-irradiated LY cells. All modified DNA bases identified in the course of this study were typical for irradiated chromatin. The total amount of initial base damage was higher in the radiation sensitive LY-S subline than in the radiation resistant LY-R subline. The repair rates of 5-OHMeUra, 5-OHCyt, 8-OHAde were similar in both cell lines, the repair rates of FapyAde and 8-OHGua were higher in the radiosensitive LY-S cell line, whereas the repair of 5-OHUra was faster in its radioresistant counterpart, the LY-R Altogether, the repair rates of the γ-ray-induced DNA base damage in LY sublines are related neither to the initial amounts of the damaged bases nor to the differential lethal or mutagenic effects of ionizing radiation in these sublines.

Keywords

EN

radiation sensitivity; : γ-rays; DNA base damage repair; L5178Y murine lymphoma sublines

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2001
• Volume: 48
• Issue: 2
• Pages: 525-533

Dates

published

2001

received

2000-01-31

revised

2001-02-2

accepted

2001-03-20

Contributors

author

Marcin Kruszewski

• Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Warszawa, Poland

author

Tomasz H Zastawny

• Department of Clinical Biochemistry, University School of Medical Sciences, Bydgoszcz, Poland

author

Irena Szumiel

• Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Warszawa, Poland

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