PL EN


Preferences help
enabled [disable] Abstract
Number of results
2001 | 48 | 2 | 525-533
Article title

Repair of γ-ray-induced base damage in L5178Y sublines is damage type-dependent and unrelated to radiation sensitivity.

Content
Title variants
Languages of publication
EN
Abstracts
EN
The L5178Y (LY) murine lymphoma sublines LY-R and LY-S are differentially sensitive to ionizing radiation. The high radiation sensitivity of LY-S cells is related to impaired rejoining of DNA double strand breaks. We found previously that the γ-ray-induced base damage is higher in the more radiosensitive LY-S subline. Here, we examine the role of the repair of ionizing radiation induced base damage in relation to the radiosensitivity difference of these sublines. We used the GS/MS technique to estimate the repair rates of six types of base damage in γ-irradiated LY cells. All modified DNA bases identified in the course of this study were typical for irradiated chromatin. The total amount of initial base damage was higher in the radiation sensitive LY-S subline than in the radiation resistant LY-R subline. The repair rates of 5-OHMeUra, 5-OHCyt, 8-OHAde were similar in both cell lines, the repair rates of FapyAde and 8-OHGua were higher in the radiosensitive LY-S cell line, whereas the repair of 5-OHUra was faster in its radioresistant counterpart, the LY-R Altogether, the repair rates of the γ-ray-induced DNA base damage in LY sublines are related neither to the initial amounts of the damaged bases nor to the differential lethal or mutagenic effects of ionizing radiation in these sublines.
Publisher

Year
Volume
48
Issue
2
Pages
525-533
Physical description
Dates
published
2001
received
2000-01-31
revised
2001-02-2
accepted
2001-03-20
Contributors
  • Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Warszawa, Poland
  • Department of Clinical Biochemistry, University School of Medical Sciences, Bydgoszcz, Poland
author
  • Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Warszawa, Poland
References
  • 1. Ward, J.F. (1999) Nature of lesions formed by ionizing radiation; in DNA Damage and Repair, vol 2: DNA Repair in Higher Eucaryotes (Nickoloff, J.A. & Hoekstra, M.F., eds.) pp. 65-84, Humana Press, Totowa, New Jersey.
  • 2. Wallace, S.S. (1998) Enzymatic processing of radiation-induced free radical damage in DNA. Radiat. Res. 150, S60-S79.
  • 3. Beer, J.Z., Budzicka, E., Niepokojczycka, E., Rosiek, O., Szumiel, I. & Walicka, M. (1983) Loss of tumorigenicity with simultaneous changes in radiosensitivity and photosensitivity during in vitro growth of L5178Y murine lymphoma cells. Cancer Res. 43, 4736-4742.
  • 4. Bouzyk, E., Buraczewska, I., Rosiek, O., Sochanowicz, B. & Szumiel, I. (1991) Hydrogen peroxide induced reproductive and interphase death in two strains of L5178Y murine lymphoma differing in radiation sensitivity. Radiat. Environ. Biophys. 30, 105-116.
  • 5. Bouzyk, E., Iwanenko, T., Jarocewicz, N., Kruszewski, M., Sochanowicz, B. & Szumiel, I. (1997) Antioxidant defense system in differentially hydrogen peroxide sensitive L5178Y sublines. Free Radic Biol. Med. 22, 697-704.
  • 6. Kruszewski, M., Green, M.H., Lowe, J.E. & Szumiel, I. (1994) DNA strand breakage, cytotoxicity and mutagenicity of hydrogen peroxide treatment at 4°C and 37°C in L5178Y sublines. Mutat. Res. 308, 233-241.
  • 7. Kruszewski, M., Green, M.H., Lowe, J.E. & Szumiel, I. (1995) Comparison of effects of iron and calcium chelators on the response of L5178Y sublines to X-rays and H2Om2. Mutat. Res. 326, 155-163.
  • 8. Wlodek, D. & Hittelman, W.N. (1987) The repair of double-strand DNA breaks correlates with radiosensitivity of L5178Y-S and L5178Y-R cells. Radiat. Res. 112, 146-155.
  • 9. Wlodek, D. & Hittelman, W.N. (1988) The relationship of DNA and chromosome damage to survival of synchronized X-irradiated L5178Y cells. II. Repair. Radiat. Res. 115, 566-575.
  • 10. Wlodek, D. & Hittelman, W.N. (1988) The relationship of DNA and chromosome damage to survival of synchronized X-irradiated L5178Y cells. I. Initial damage. Radiat. Res. 115, 550-565.
  • 11. Kapiszewska, M., Szumiel, I. & Lange, C.S. (1992) Damage at two levels of DNA folding measured by fluorescent halo technique in X-irradiated L5178Y-R and L5178Y-S cells. I. Initial lesions. Radiat. Environ. Biophys. 31, 311-322.
  • 12. Szumiel, I., Kapiszewska, M., Kruszewski, M., Iwanenko, T. & Lange, C.S. (1995) Content of iron and copper in the nuclei and induction of pH 9-labile lesions in L5178Y sublines inversely cross-sensitive to H2O2 and X-rays. Radiat. Environ. Biophys. 34, 113-119.
  • 13. Zastawny, T.H., Kruszewski, M. & Olinski, R. (1998) Ionizing radiation and hydrogen peroxide induced oxidative DNA base damage in two L5178Y cell lines. Free Radic Biol. Med. 24, 1250-1255.
  • 14. Mori, T. & Dizdaroglu, M. (1994) Ionizing radiation causes greater DNA base damage in radiation-sensitive mutant M10 cells than in parent mouse lymphoma L5178Y cells. Radiat. Res. 140, 85-90.
  • 15. Szumiel, I. (1979) Response of two strains of L5178Y cells to cis-dichlorobis (cyclopentylamine)platinum(II). I. Cross-sensitivity to cis- PAD and UV light. Chem. Biol. Interact. 24, 51-72.
  • 16. Mee, L.K. & Adelstein, S.J. (1981) Predominance of core histones in formation of DNA- protein crosslinks in gamma-irradiated chromatin. Proc. Natl. Acad. Sci. U.S.A. 78, 2194- 2198.
  • 17. Dizdaroglu, M. (1994) Chemical determination of oxidative DNA damage by gas chromatography-mass spectrometry. Methods Enzymol. 234, 3-16.
  • 18. Halliwell, B. & Dizdaroglu, M. (1992) The measurement of oxidative damage to DNA by HPLC and GC/MS techniques. Free Radic Res. Commun. 16, 75-87.
  • 19. Ward, J.F. (1995) Radiation mutagenesis: The initial DNA lesions responsible. Radiat. Res. 142, 362-368.
  • 20. Dizdaroglu, M., Rao, G., Halliwell, B. & Gajewski, E. (1991) Damage to the DNA bases in mammalian chromatin by hydrogen peroxide in the presence of ferric and cupric ions. Arch. Biochem. Biophys. 285, 317-324.
  • 21. Gajewski, E., Rao, G., Nackerdien, Z. & Dizdaroglu, M. (1990) Modification of DNA bases in mammalian chromatin by radiation-generated free radicals. Biochemistry 29, 7876-7882.
  • 22. Martins, E.A., Chubatsu, L.S. & Meneghini, R. (1991) Role of antioxidants in protecting cellular DNA from damage by oxidative stress. Mutat. Res. 250, 95-101.
  • 23. Meneghini, R. (1997) Iron homeostasis, oxidative stress, and DNA damage. Free Radic Biol. Med. 23, 783-792.
  • 24. Aldridge, D.R. & Radford, I.R. (1998) Explaining differences in sensitivity to killing by ionizing radiation between human lymphoid cell lines. Cancer Res. 58, 2817-2824.
  • 25. Laval, J., Jurado, J., Saparbaev, M. & Sidorkina, O. (1998) Antimutagenic role of base-excision repair enzymes upon free radical-induced DNA damage. Mutat. Res. 402, 93-102.
  • 26. Beckman, K.B. & Ames, B.N. (1997) Oxidative decay of DNA. J. Biol. Chem. 272, 19633- 19636.
  • 27. Loft, S. & Poulsen, H.E. (1996) Cancer risk and oxidative DNA damage in man. J. Mol. Med. 74, 297-312.
  • 28. Bohr, V.A. & Anson, R.M. (1995) DNA damage, mutation and fine structure DNA repair in aging. Mutat. Res. 338, 25-34.
  • 29. Evans, H.H., Ricanati, M., Di Salvo, C., Horng, M.F. & Mencl, J. (1991) Induction of multilocus lesions by UVC-radiation in mouse L5178Y lymphoblasts. Mutation Res. 251, 79-90.
  • 30. Yi, P.N., Evans, H.H., Beer, J.Z. & Rha, C.K. (1994) Relationships between mitotic delay and the dose rate of X radiation. Radiat. Res. 140, 387-392.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv48i2p525kz
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.