Sequence determination and analysis of S-adenosyl-L-homocysteine hydrolase from yellow lupine (Lupinus luteus).

• Authors: Krzysztof Brzeziński , Robert Janowski , Jan Podkowiński , Mariusz Jaskólski
• Languages of publication: EN

Abstracts

EN

The coding sequences of two S-adenosyl-L-homocysteine hydrolases (SAHases) were identified in yellow lupine by screenig of a cDNA library. One of them, corresponding to the complete protein, was sequenced and compared with 52 other SAHase sequences. Phylogenetic analysis of these proteins identified three groups of the enzymes. Group A comprises only bacterial sequences. Group B is subdivided into two subgroups, one of which (B1) is formed by animal sequences. Subgroup B2 consist of two distinct clusters, B2a and B2b. Cluster B2b comprises all known plant sequences, including the yellow lupine enzyme, which are distinguished by a 50-residue insert. Group C is heterogeneous and contains SAHases from Archaea as well as a new class of animal enzymes, distinctly different from those in group B1.

Keywords

EN

Southern blot; S-adenosyl-L-homocysteinase; Northern blot; biological methylation; screening of cDNA library; phylogeny

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2001
• Volume: 48
• Issue: 2
• Pages: 477-483

Dates

published

2001

received

2001-02-14

revised

2001-04-25

accepted

2001-05-23

Contributors

author

Krzysztof Brzeziński

• Department of Crystallography, Faculty of Chemistry, Adam Mickiewicz University, Poznań, Poland

author

Robert Janowski

• Department of Crystallography, Faculty of Chemistry, Adam Mickiewicz University, Poznań, Poland

author

Jan Podkowiński

• >Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland

author

Mariusz Jaskólski

• Department of Crystallography, Faculty of Chemistry, Adam Mickiewicz University, Poznań, Poland

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