Ribozymes of the hepatitis delta virus: Recent findings on their structure, mechanism of catalysis and possible applications.

• Authors: Jerzy Ciesiołka , Jan Wrzesinski , Michał Łęgiewicz , Barbara Smólska , Mariola Dutkiewicz
• Languages of publication: EN

Abstracts

EN

Although the delta ribozymes have been studied for more than ten years the most important information concerning their structure and mechanism of catalysis were only obtained very recently. The crystal structure of the genomic delta ribozyme turns out to be an excellent example of the extraordinary properties of RNA molecules to fold into uniquely compact structures. Details of the X-ray structure have greatly stimulated further studies on the folding of the ribozymes into functionally active molecules as well as on the mechanism of RNA catalysis. The ability of the delta ribozymes to carry out general acid-base catalysis by nucleotide side chains has been assumed in two proposed mechanisms of self-cleavage. Recently, considerable progress has been also made in characterizing the catalytic properties of trans-acting ribozyme variants that are potentially attractive tools in the strategy of directed RNA degradation.

Keywords

EN

RNA structure; RNA catalysis; HDV; hepatitis delta virus; ribozyme

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2001
• Volume: 48
• Issue: 2
• Pages: 409-418

Dates

published

2001

received

2001-02-14

revised

2001-04-7

accepted

2001-05-14

Contributors

author

Jerzy Ciesiołka

• Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland

author

Jan Wrzesinski

• Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland

author

Michał Łęgiewicz

• Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland

author

Barbara Smólska

• Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland

author

Mariola Dutkiewicz

• Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland

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