Editing of plant mitochondrial transfer RNAs

• Authors: Julien Fey , Jacques H Weil , K Tomita , Anne Cosset , André Dietrich , Ian Small , Laurence Maréchal-Drouard
• Languages of publication: EN

Abstracts

EN

Editing in plant mitochondria consists in C to U changes and mainly affects messenger RNAs, thus providing the correct genetic information for the biosynthesis of mitochondrial (mt) proteins. But editing can also affect some of the plant mt tRNAs encoded by the mt genome. In dicots, a C to U editing event corrects a C:A mismatch into a U:A base-pair in the acceptor stem of mt tRNAPhe (GAA). In larch mitochondria, three C to U editing events restore U:A base-pairs in the acceptor stem, D stem and anticodon stem, respectively, of mt tRNAHis (GUG). For both these mt tRNAs editing of the precursors is a prerequisite for their processing into mature tRNAs. In potato mt tRNACys (GCA), editing converts a C28:U42 mismatch in the anticodon stem into a U28:U42 non-canonical base-pair, and reverse transcriptase minisequencing has shown that the mature mt tRNACys is fully edited. In the bryophyte Marchantia polymorpha this U residue is encoded in the mt genome and evolutionary studies suggest that restoration of the U28 residue is necessary when it is not encoded in the gene. However, in vitro studies have shown that neither processing of the precursor nor aminoacylation of tRNACys requires C to U editing at this position. But sequencing of the purified mt tRNACys has shown that is present at position 28, indicating that C to U editing is a prerequisite for the subsequent isomerization of U into Ψ at position 28.

Keywords

EN

tRNA; pseudouridine; plant mitochondria; processing; RNA editing

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2001
• Volume: 48
• Issue: 2
• Pages: 383-389

Dates

published

2001

received

2001-02-14

accepted

2001-04-15

Contributors

author

Julien Fey

• Institut de Biologie Moléculaire des Plantes, CNRS, Strasbourg, France

author

Jacques H Weil

• Institut de Biologie Moléculaire des Plantes, CNRS, Strasbourg, France

author

K Tomita

• Institut de Biologie Moléculaire des Plantes, CNRS, Strasbourg, France

author

Anne Cosset

• Institut de Biologie Moléculaire des Plantes, CNRS, Strasbourg, France

author

André Dietrich

• Institut de Biologie Moléculaire des Plantes, CNRS, Strasbourg, France

author

Ian Small

• Station de Génétique et d'Amelioration des Plantes, INRA, Versailles, France

author

Laurence Maréchal-Drouard

• Institut de Biologie Moléculaire des Plantes, CNRS, Strasbourg, France

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