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2001 | 48 | 2 | 383-389
Article title

Editing of plant mitochondrial transfer RNAs

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EN
Abstracts
EN
Editing in plant mitochondria consists in C to U changes and mainly affects messenger RNAs, thus providing the correct genetic information for the biosynthesis of mitochondrial (mt) proteins. But editing can also affect some of the plant mt tRNAs encoded by the mt genome. In dicots, a C to U editing event corrects a C:A mismatch into a U:A base-pair in the acceptor stem of mt tRNAPhe (GAA). In larch mitochondria, three C to U editing events restore U:A base-pairs in the acceptor stem, D stem and anticodon stem, respectively, of mt tRNAHis (GUG). For both these mt tRNAs editing of the precursors is a prerequisite for their processing into mature tRNAs. In potato mt tRNACys (GCA), editing converts a C28:U42 mismatch in the anticodon stem into a U28:U42 non-canonical base-pair, and reverse transcriptase minisequencing has shown that the mature mt tRNACys is fully edited. In the bryophyte Marchantia polymorpha this U residue is encoded in the mt genome and evolutionary studies suggest that restoration of the U28 residue is necessary when it is not encoded in the gene. However, in vitro studies have shown that neither processing of the precursor nor aminoacylation of tRNACys requires C to U editing at this position. But sequencing of the purified mt tRNACys has shown that is present at position 28, indicating that C to U editing is a prerequisite for the subsequent isomerization of U into Ψ at position 28.
Publisher

Year
Volume
48
Issue
2
Pages
383-389
Physical description
Dates
published
2001
received
2001-02-14
accepted
2001-04-15
Contributors
author
  • Institut de Biologie Moléculaire des Plantes, CNRS, Strasbourg, France
  • Institut de Biologie Moléculaire des Plantes, CNRS, Strasbourg, France
author
  • Institut de Biologie Moléculaire des Plantes, CNRS, Strasbourg, France
author
  • Institut de Biologie Moléculaire des Plantes, CNRS, Strasbourg, France
  • Institut de Biologie Moléculaire des Plantes, CNRS, Strasbourg, France
author
  • Station de Génétique et d'Amelioration des Plantes, INRA, Versailles, France
  • Institut de Biologie Moléculaire des Plantes, CNRS, Strasbourg, France
References
  • Arnez, J. & Steitz, T. (1994) Crystal structure of unmodified tRNAGln complexed with glutaminyl-tRNA synthetase and ATP suggests a possible role for pseudouridines in stabilization of RNA structure. Biochemistry 33, 7560-7567.
  • Binder, S., Marchfelder, A. & Brennicke, A. (1994) RNA editing of tRNAPhe and tRNACys in mitochondria of Oenothera berteriana is initiated in precursor molecules. Mol. Gen. Genet. 244, 67-74.
  • Börner, G., Morl, M., Wissinger, B., Brennicke, A. & Schmelzer, C. (1995) RNA editing of a group II intron in Oenothera as a prerequisite for splicing. Mol. Gen. Genet. 246, 739-744.
  • Bourara, K., Litvak, S. & Araya, A. (2000) Generation of G-to-A and C-to-U changes in HIV-1 transcripts by RNA editing. Science 289, 1564-1566.
  • Davis, D. (1995) Stabilization of RNA stacking by pseudouridine. Nucleic Acids Res. 23, 5020-5026.
  • Davis, D. & Poulter, C.D. (1991) 1H-15N NMR studies of Escherichia coli tRNAPhe from hisT mutants: A structural role for pseudouridine. Biochemistry 30, 4223-4231.
  • Durant, P.C. & Davis, D.R. (1999) Stabilization of the anticodon stem-loop of tRNALys,3 by an A+-C base-pair and by pseudouridine. J. Mol. Biol. 285, 115-131.
  • Faivre-Nitschke, S.E., Grienenberger, J.M. & Gualberto, J. (2000) A prokaryotic-type cytidine deaminase from Arabidopsis thaliana - Gene expression and functional characterization. Eur. J. Biochem. 263, 896-903.
  • Fey, J., Tomita, K., Bergdoll, M. & Maréchal- Drouard, L. (2000) Evolutionary and functional aspects of C-to-U editing at position 28 of tRNACys (GCA) in plant mitochondria. RNA 6, 470-474.
  • Giegé, P. & Brennicke, A. (1999) RNA editing in Arabidopsis mitochondria effects 441 C to U changes in ORFs. Proc. Natl. Acad. Sci. U.S.A. 96, 15324-15329.
  • Hiesel, R., Combettes, B. & Brennicke, A. (1994) Evidence of RNA editing in mitochondria of all major groups of land plants except the bryophyta. Proc. Natl. Acad. Sci. U.S.A. 91, 629-633.
  • Kunzmann, A., Brennicke, A. & Marchfelder, A. (1998) 5' End maturation and RNA editing have to precede tRNA 3' processing in plant mitochondria. Proc. Natl. Acad. Sci. U.S.A. 95, 108-113.
  • Maier, R., Zeitz, P., Kossel, H., Bonnard, G., Gualberto, J. & Grienenberger, J.M. (1996) RNA editing in plant mitochondria and chloroplasts. Plant Mol. Biol. 32, 343-365.
  • Malek, O., Lättig, K., Hiesel, R., Brennicke, A. & Knoop, V. (1996) RNA editing in bryophytes and a molecular phytogeny of land plants. EMBO J. 15, 1403-1411.
  • Marchfelder, A., Brennicke, A. & Binder, S. (1996) RNA editing is required for efficient excision of tRNA(Phe) from precursors in plant mitochondria. J. Biol. Chem. 271, 1898-1903.
  • Maréchal-Drouard, L., Cosset, A., Remade, C., Ramamonjisoa, D. & Dietrich, A. (1996a) A single editing event is a prerequisite for efficient processing of potato mitochondrial phenylalanine tRNA. Mol. Cell. Biol. 16, 3504-3510.
  • Maréchal-Drouard, L., Kumar, R., Remade, C. & Small, I. (1996b) RNA editing of larch mitochondrial tRNA(His) precursors is a prerequisite for processing. Nucleic Acids Res. 24, 3229-3234.
  • Motorin, Y.A., Auxilien, S. & Grosjean, H. (1996) Potential Editing of Cytidine to Dihydrouridine in E. coli tRNASer (GGA). Proc. EMBO Workshop on RNA Editing, p. 75.
  • Price, D. & Gray, M. (1998) Editing of tRNA; in Modification and Editing of RNA (Grosjean, H., ed.) pp. 289-305, ASM Press, Washington.
  • Schock, I., Maréchal-Drouard, L., Marchfelder, A. & Binder, S. (1998) Processing of plant mitochondrial tRNAGly and tRNASer (GCU) is independent of RNA editing. Mol. Gen. Genet. 257, 554-560.
  • Smith, H., Gott, J. & Hanson, M. (1997) A guide to RNA editing. RNA 3, 1105-1123.
  • Westhof, E. & Moras, D. (1988) Hydration of transfer RNA molecules: A crystallographic study. Biochimie 70, 145-165.
  • Yu, W. & Schuster, W. (1995) Evidence for a site-specific cytidine deamination reaction involved in C to U RNA editing of plant mitochondria. J. Biol. Chem. 270, 18227-18233.
Document Type
Publication order reference
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YADDA identifier
bwmeta1.element.bwnjournal-article-abpv48i2p383kz
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