nod Genes and Nod signals and the evolution of the rhizobium legume symbiosis.

• Authors: Frédéric Debellé , Lionel Moulin , Brigitte Mangin , Jean Dénarié , Catherine Boivin
• Languages of publication: EN

Abstracts

EN

The establishment of the nitrogen-fixing symbiosis between rhizobia and legumes requires an exchange of signals between the two partners. In response to flavonoids excreted by the host plant, rhizobia synthesize Nod factors (NFs) which elicit, at very low concentrations and in a specific manner, various symbiotic responses on the roots of the legume hosts. NFs from several rhizobial species have been characterized. They all are lipo-chitooligosaccharides, consisting of a backbone of generally four or five glucosamine residues N-acylated at the non-reducing end, and carrying various O-substituents. The N-acyl chain and the other substituents are important determinants of the rhizobial host specificity. A number of nodulation genes which specify the synthesis of NFs have been identified. All rhizobia, in spite of their diversity, possess conserved nodABC genes responsible for the synthesis of the N-acylated oligosaccharide core of NFs, which suggests that these genes are of a monophyletic origin. Other genes, the host specific nod genes, specify the substitutions of NFs. The central role of NFs and nod genes in the Rhizobium-legume symbiosis suggests that these factors could be used as molecular markers to study the evolution of this symbiosis. We have studied a number of NFs which are N-acylated by α,β-unsaturated fatty acids. We found that the ability to synthesize such NFs does not correlate with taxonomic position of the rhizobia. However, all rhizobia that produce NFs such nodulate plants belonging to related tribes of legumes, the Trifolieae, Vicieae, and Galegeae, all of them being members of the so-called galegoid group. This suggests that the ability to recognize the NFs with α,β-unsaturated fatty acids is limited to this group of legumes, and thus might have appeared only once in the course of legume evolution, in the galegoid phylum.

Keywords

EN

evolution; symbiosis; nodulation; legume; rhizobium; Nod factors

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2001
• Volume: 48
• Issue: 2
• Pages: 359-365

Dates

published

2001

received

2001-02-14

Contributors

author

Frédéric Debellé

• LBMRPM INRA-CNRS, BP27 31326 Castanet Tolosan Cedex, France

author

Lionel Moulin

• LSTM-IRD, BP5035 34032 Montpellier Cedex, France

author

Brigitte Mangin

• BIA-INRA, BP27 31326 Castanet Tolosan Cedex, France

author

Jean Dénarié

• LBMRPM INRA-CNRS, BP27 31326 Castanet Tolosan Cedex, France

author

Catherine Boivin

• LSTM-IRD, BP5035 34032 Montpellier Cedex, France

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