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2001 | 48 | 1 | 257-269
Article title

The interactions of phthalocyanines with stimulated and resting human peripheral blood mononuclear cells.

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The interactions of two metal-free phthalocyanines [(H2Pc) and Solar Pc (with four peripherical groups: SO2N(CH2CH2OH)2)] and of one metal substituted dye (CoPc) with resting and stimulated human peripheral blood mononuclear cells (PBMC) were compared. The absorption, fluorescence, photoacoustic and EPR spectra of both resting cells and cells stimulated by phytohaemagglutinin, incubated in dimethyl sulfoxide (DMSO) with very low or 95% water content and with or without dye addition, were measured. The fate of the light absorbed by the samples was investigated. It is known that singlet oxygen production is crucial for photodynamic action of dyes. Thermal deactivation and luminescence emission compete with this process, so investigation of these alternative paths of sensitizer deactivation provides information about photodynamic action. The incorporation of the investigated dyes into cells and the perturbation of the cell structure caused by the dyes, the incubation solvent and the activator were investigated by comparing the spectral properties of PBMC before and after stimulation and incubation. Incubation of the cells for 1 h in a solution of Solar Pc in 99.5% aqueous DMSO, resulted in an efficient dye incorporation which was highly selective. Solar Pc being introduced much more efficiently into stimulated cells than into resting cells.
Physical description
  • Institute of Physics, Poznań University of Technology, Poznań, Poland
  • Institute of Physics, Poznań University of Technology, Poznań, Poland
  • Institute of Physics, Poznań University of Technology, Poznań, Poland
  • ZECASIN, Bucharest, Romania
  • Department of Biology and Environmental Studies, K. Marcinkowski University of Medical Sciences, Poznań, Poland
  • Department of Biology and Environmental Studies, K. Marcinkowski University of Medical Sciences, Poznań, Poland
  • 1. Rosenthal, I. (1991) Phthalocyanines as photodynamic sensitizers. Photochem. Photobiol. 53, 859-870.
  • 2. Frąckowiak, D., Planner, A., Ion, R.M. & Wiktorowicz, K. (1997) Incorporation of dye in resting and stimulated leukocytes; in Near-Infrared Dyes for High Technology Applications (Daehne, S., Resch-Gennger, U. & Wolfbais, O.A., eds.), pp. 67-114, Kluwer Academic Press, Dodrecht.
  • 3. Ion, R.M., Planner, A., Wiktorowicz, K. & Frąckowiak, D. (1998) The incorporation of various porphyrins into blood cells measured via flow cytometry, absorption and emission spectroscopy. Acta Biochim. Polon. 45, 833-845.
  • 4. Boyle, R.W. & Dolphin, D. (1996) Structure and biodistribution relationships of photodynamic sensitizers. Photochem. Photobiol. 64, 469-485.
  • 5. Assour, J.M. & Harrison, S.M. (1964) On origin of unpaired electrons in metal-free phthalocyanine. J. Phys. Chem. 68, 872-876.
  • 6. Frąckowiak, D., Niedbalska, M. & Wiktorowicz, K. (1996) Influence of mitogenetic activators on the structure of leukocytes. J. Photochem. Photobiol. B: Biology 33, 45-50.
  • 7. Moser, F.H. & Thomas, A.L. (1963) Phthalocyanine compounds, 105 pp., Reinhold Publishing Corporation, New York.
  • 8. Monahan, A.R., Brado, J.A. & De Luca, A.F. (1972) The association of copper (II) vanadyl and zinc (II) 4, 4'', 4'', 4''' tetraalkylphthalocyanines dyes in benzene. J. Phys. Chem. 76, 1994-1996.
  • 9. Rollmann, L.D. & Iwamoto, R.T. (1968) Electrochemistry, EPR and visible spectra of Co, Ni, Cu and metalphthalocyanines in DMSO. J. Am. Chem. Soc. 90, 1455-1463.
  • 10. Ion, R.M., Yamaz, I. & Bekaroglu, O. (1999) Supermolecular assemblies of pirydil porphyrin and diazadithya phthalocyanine. Serb. J. Chem. Soc. 64, 453-462.
  • 11. Ion, R.M. (1999) Implications of supermolecular aggregates of MeP and MePc in PDT. Int. Conf. Bioorganic Chemistry. Greece, 123.
  • 12. Frąckowiak, D., Wiktorowicz, K., Cofta, J., Niedbalska, M. & Latosinska, M. (1995) Incorporation of stilbazolium merocyanines into resting and stimulated mononuclear leukocytes. Acta Biochim. Polon. 42, 61-68.
  • 13. Wiktorowicz, K., Niedbalska, M., Planner, A. & Frąckowiak, D. (1995) Incorporation of stilbazolium merocyanines into human leukocytes measured by flow cytometry. Acta Biochim. Polon. 42, 333-338.
  • 14. Moan, J. & Anholt, H. (1990) Phthalocyanine fluorescence in tumor during photodynamic therapy. Photochem. Photobiol. 51, 379-381.
  • 15. Frąckowiak, D., Dudkowiak, A., Cegielski, R., Planner, A. & Schultz, C. (1995) Optical properties of the purple bacteria reaction centres immobilized in polymer film. Photosynthetica 31, 283-299.
  • 16. Ducharm, D., Tessier, A. & Leblanc, R.M. (1979) Design and characteristics of a cell for photoacoustic spectroscopy of condensed matter. Rev. Sci. Instr. 50, 1461-1462.
  • 17. Ouzafe, M., Poulet, P. & Chambron, J. (1992) Photoacoustic detection of triplet state and singlet oxygen in highly absorbing samples. Photochem. Photobiol. 55, 491-503.
  • 18. Lukyanets, E.A. (1998) NIR photosensitizers in photodynamic therapy; in Near-Infrared Dyes for High Technology Applications (Daehne, S., Resch- Genger, U., Wolfbeis, O.S., eds.) pp. 307-324, Kluwer Acad. Publ.
  • 19. Assour, J.M. & Kahn, W.K. (1965) Electron spin resonance of α and β-cobalt phthalocyanine. J. Am. Chem. Soc. 87, 207-212.
  • 20. Rollman, L.D. & Iwamoto, R.T. (1968) Electrochemistry, electron paramagnetic resonance, and visible spectra of cobalt, nickel, copper and metal-free phthalocyanines in dimethyl sulfoxide. J. Am. Chem. Soc. 90, 1455-1463.
  • 21. Oleinick, N.L., Atunez, A.R, Clay, M.E, Rihter, B.D. & Kenney, M.E. (1996), New phthalocyanine photosensitizers for photodynamic therapy. Photochem. Photobiol. 57, 242-247.
  • 22. He, J., Larkin, H.E., Ying-Syi Li, Richter, B.D., Zaidi, S.I.A., Rodgers, M.A.J., Mukhtar, H., Kenney, M.E. & Oleinick, N.L. (1997) The synthesis, photophysical and photobiological properties and in vivo structure-activity relationship of set of silicon phthalocyanine PDT photosensitizers. Photochem. Photobiol. 65, 581-586.
  • 23. Anderson, C.Y., Freye, K., Tubesing, K.A., Ying-Syi Li, Kenney, M.E., Mukhtar, H. & Elmets, C.A. (1998) Comparative analysis of silicon phthalocyanine photosensitizers for in vivo photodynamic therapy of RIF-1 tumors in C3H mice. Photochem. Photobiol. 67, 332-336.
  • 24. Takamura, T., Ohta, N., Nakajima, S. & Sekata, I. (1989) Critical importance of the triplet lifetime of photosensitizer in photodynamic therapy of tumor. Photochem. Photobiol. 50, 339-344.
  • 25. Gomez, E.R., Calvalho, A.P. & Duarte, C.B. (1999) Effect of pre-incubation time and fluence rate on cell variability after photodynamic treatment with phthalocyanines. Photochem. Photobiol. Abstracts of the 26-th Annual Meeting of the American Society for Photobiology, pp. 99S-100S.
  • 26. Heintzelman, D.L., Lotan, R. & Richards- Kortum, R.R. (2000) Characterization of the autofluorescence of polymorphonuclear leukocytes, monocytes and cervical epithelial cancer cells for improved spectroscopic discrimination of inflammation from dysplasia. Photochem. Photobiol. 71, 327-332.
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