Expression of bovine leukemia virus protein p24 in Escherichia coli and its use in the immunoblotting assay.

• Authors: Leokadia Bicka , Jacek Kuźmak , Bożena Kozaczyńska , Andrzej Płucienniczak , Anna Skorupska
• Languages of publication: EN

Abstracts

EN

The gag gene encoded protein, p24 of bovine leukemia virus (BLV), was cloned and expressed as thioredoxin-6xHis-p24 protein in Escherichia coli. The bacterial cells carrying plasmid pT7THis-p24 expressed the protein of 38 kDa that was detected by immunoblotting analysis using anti-p24 monoclonal antibodies and sera from BLV infected cattle and sheep. The purified p24 fusion protein was shown to be sensitive and specific for detection of BLV antibodies in the infected cattle.

Keywords

EN

bovine leukemia virus; fusion protein p24; immunoblotting assay; gag gene cloning

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2001
• Volume: 48
• Issue: 1
• Pages: 227-232

Dates

published

2001

received

2000-08-2

accepted

2001-02-04

revised

2001-02-9

Contributors

author

Leokadia Bicka

• Department of Biochemistry, National Veterinary Research Institute, Puławy, Poland

author

Jacek Kuźmak

• Department of Biochemistry, National Veterinary Research Institute, Puławy, Poland

author

Bożena Kozaczyńska

• Department of Biochemistry, National Veterinary Research Institute, Puławy, Poland

author

Andrzej Płucienniczak

• Institute of Biotechnology and Antibiotics, Warszawa, Poland

author

Anna Skorupska

• Department of Microbiology, M. Curie-Skłodowska University, Lublin, Poland

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