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2001 | 48 | 1 | 227-232
Article title

Expression of bovine leukemia virus protein p24 in Escherichia coli and its use in the immunoblotting assay.

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EN
Abstracts
EN
The gag gene encoded protein, p24 of bovine leukemia virus (BLV), was cloned and expressed as thioredoxin-6xHis-p24 protein in Escherichia coli. The bacterial cells carrying plasmid pT7THis-p24 expressed the protein of 38 kDa that was detected by immunoblotting analysis using anti-p24 monoclonal antibodies and sera from BLV infected cattle and sheep. The purified p24 fusion protein was shown to be sensitive and specific for detection of BLV antibodies in the infected cattle.
Publisher

Year
Volume
48
Issue
1
Pages
227-232
Physical description
Dates
published
2001
received
2000-08-2
accepted
2001-02-04
revised
2001-02-9
Contributors
  • Department of Biochemistry, National Veterinary Research Institute, Puławy, Poland
author
  • Department of Biochemistry, National Veterinary Research Institute, Puławy, Poland
  • Department of Biochemistry, National Veterinary Research Institute, Puławy, Poland
  • Institute of Biotechnology and Antibiotics, Warszawa, Poland
  • Department of Microbiology, M. Curie-Skłodowska University, Lublin, Poland
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv48i1p227kz
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