A novel isoform of human lymphoid enhancer-binding factor-1 (LEF-1) gene transcript encodes a protein devoid of HMG domain and nuclear localization signal.

• Authors: Agnieszka Kobielak , Krzysztof Kobielak , Wieslaw H Trzeciak
• Languages of publication: EN

Abstracts

EN

Lymphoid enhancer-binding factor-1 (LEF-1), a member of the high mobility group (HMG) family of proteins, regulates expression of T-cell receptor-α gene and is one of the key regulatory molecules in the epithelial-mesenchymal interactions during embryonic development. Among others, LEF-1 regulates expression of cytokeratin genes involved in formation of hair follicles and the gene encoding the cell-adhesion molecule E-cadherin. Transcription factor LEF-1, which acts as a dimer, binds β-catenin and is involved in signal transduction by the wnt pathway. We have cloned and sequenced a novel isoform of human LEF-1 gene transcript. This isoform encodes a truncated protein devoid of HMG domain and nuclear localization signal but retaining β-catenin binding domain. This isoform might either act in a dominant-negative manner by interfering with native LEF-1, or might bind β-catenin in the cytosol, which would result in attenuation of the signals transmitted by theLEF-b-catenin pathway.

Keywords

EN

human LEF-1; novel transcript isoform; HMG domain

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2001
• Volume: 48
• Issue: 1
• Pages: 221-226

Dates

published

2001

received

2001-01-29

revised

2001-02-1

Contributors

author

Agnieszka Kobielak

• Department of Biochemistry and Molecular Biology, University of Medical Sciences, Poznań, Poland

author

Krzysztof Kobielak

• Department of Biochemistry and Molecular Biology, University of Medical Sciences, Poznań, Poland

author

Wieslaw H Trzeciak

• Department of Biochemistry and Molecular Biology, University of Medical Sciences, Poznań, Poland

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