Application of genetic semihomology algorithm to theoretical studies on various protein families.

• Authors: Jacek Leluk , Beata Hanus-Lorenz , Aleksander F Sikorski
• Languages of publication: EN

Abstracts

EN

Several protein families of different nature were studied for genetic relationship, correct alignment at non-homologous fragments, optimal sequence consensus construction, and confirmation of their actual relevance. A comparison of the genetic semihomology approach with statistical approaches indicates a high accuracy and cognition significance of the former. This is particularly pronounced in the study of related proteins that show a low degree of homology. The sequence multiple alignments were verified and corrected with respect to the questionable, non-homologous fragments. The verified alignments were the basis for consensus sequence formation. The frequency of six-codon amino acids occurrence versus position variability was studied and their possible role in amino acid mutational exchange at variable positions is discussed.

Keywords

EN

multiple alignment; cryptic mutations; genetic semihomology; sequence consensus

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2001
• Volume: 48
• Issue: 1
• Pages: 21-33

Dates

published

2001

received

2000-12-29

revised

2001-01-31

accepted

2001-03-5

Contributors

author

Jacek Leluk

• Institute of Biochemistry and Molecular Biology, University of Wrocław, Wrocław, Poland

author

Beata Hanus-Lorenz

• Institute of Biochemistry and Molecular Biology, University of Wrocław, Wrocław, Poland

author

Aleksander F Sikorski

• Institute of Biochemistry and Molecular Biology, University of Wrocław, Wrocław, Poland

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