The flavonoids, quercetin and isorhamnetin 3-O-acylglucosides diminish neutrophil oxidative metabolism and lipid peroxidation.

• Authors: Małgorzata Zielińska , Artur Kostrzewa , Ewa Ignatowicz , Jaromir Budzianowski
• Languages of publication: EN

Abstracts

EN

Two natural flavonoids, quercetin and isorhamnetin 3-O-acylglucosides, were examined for their inhibitory influence on the in vitro production and release of reactive oxygen species in polymorphonuclear neutrophils (PMNs). The generation of superoxide radical, hydrogen peroxide and hypochlorous acid were measured by, respectively, cytochrome c reduction, dichlorofluorescin oxidation and taurine chlorination. Membrane lipid oxidation was studied by the thiobarbituric acid method in mouse spleen microsomes. The addition of flavonoids at the concentration range 1-100 μM inhibited PMNs oxidative metabolism and lipid peroxidation in a dose-dependent manner. The results suggest that these flavonoids suppress the oxidative burst of PMNs and protect membranes against lipid peroxidation.

Keywords

EN

flow cytometry; respiratory burst; polymorphonuclear neutrophils; reactive oxygen species; lipid peroxidation; flavonoids

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2001
• Volume: 48
• Issue: 1
• Pages: 183-189

Dates

published

2001

received

2000-09-20

revised

2001-01-8

accepted

2001-02-13

Contributors

author

Małgorzata Zielińska

• Department of Pharmaceutical Biochemistry, Karol Marcinkowski University of Medical Sciences, Poznań, Poland

author

Artur Kostrzewa

• Department of Immunodiagnostics, Karol Marcinkowski University of Medical Sciences, Poznań, Poland

author

Ewa Ignatowicz

• Department of Pharmaceutical Biochemistry, Karol Marcinkowski University of Medical Sciences, Poznań, Poland

author

Jaromir Budzianowski

• Department of Pharmaceutical Botany, Karol Marcinkowski University of Medical Sciences, Poznań, Poland

References

• 1. Edwards, S.W. (1994) The respiratory burst: The generation of reactive oxygen metabolites and their role in microbial killing; in: Biochemistry and Physiology of Neutrophil; pp. 149-179, Cambridge University Press, Cambridge.
• 2. Hollman, P.C.H. & Katan, M.B. (1999) Dietary flavonoids: Intake, health effects and bioavailability. Food Chem. Toxicol. 37, 937-942.
• 3. Halliwell, B. (1996) Oxidative stress, nutrition and health. Experimental strategies for optimization of nutritional antioxidant intake in humans. Free Radic Res. 25, 57-74.
• 4. Skaper, S.D., Fabris, M., Ferrari, V., Carbonare, M.D. & Leon, A. (1997) Quercetin protects cutaneous tissue-associated cell types including sensory neurons from oxidative stress induced by glutathione depletion: Cooperative effects of ascorbic acid. Free Radic. Biol. Med. 22, 669-678.
• 5. Catapano, A.L. (1997) Antioxidant effect of flavonoids. Angiology 48, 39-44.
• 6. Cao, G., Sofic, E. & Prior, R.L. (1997) Antioxidant and prooxidant behavior of flavonoids: Structure-activity relationships. Free Radic. Biol. Med. 22, 749-760.
• 7. Cotelle, N., Bernier, J.L., Catteau, J.P., Pommery, J., Vallet, J.C. & Gaydou, E.M. (1996) Antioxidant properties of hydroxy-flavones. Free Radic. Biol. Med. 20, 35-43.
• 8. Budzianowski, J., Skrzypczak, L. & Wesołowska, M. (1990) Flavonoids and leiocarposide in four Solidago taxa. Sci. Pharma. 58, 15-23.
• 9. Metcalf, J.A., Gallin, J.I., Nausseef, W.M. & Root, R.K. (1986) Preparation of soluble stimuli; in Laboratory Manual of Neutrophil Function; p. 23, Raven Press, New York.
• 10. Ferrante, H. & Thong, Y.H. (1978) A rapid one-step procedure for purification of mononuclear and polymorphonuclear leukocytes from human blood using a modification of the Hypaque-Ficoll technique. J. Immunol. Methods 24, 389-393.
• 11. Gnojkowski, J., Baer-Dubowska, W., Klimek, D. & Chmiel, J. (1984) Effect of toluidines on drug metabolising enzymes in rat liver, kidney and lung. Toxicology 32, 335-342.
• 12. Lowry, O.H., Rosebrough, N.J., Farr, A. & Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275.
• 13. Cohen, H.J. & Chovaniec, M.E. (1978) Superoxide generation by digitionin-stimulated guinea pig granulocytes. A basis for continuous assay for monitoring superoxide production and for study of the activation of the generating system. J. Clin. Invest. 61, 1081-1087.
• 14. Gelder, B.F. & Slater, E.C. (1962) The extinction of cytochrome c. Biochim. Biophys. Acta 58, 593-595.
• 15. Weiss, S.J., Klein, R., Slivka, A. & Wei, M. (1982) Chlorination of taurine by human neutrophils. Evidence for hypochlorous acid generation. J. Clin. Invest. 70, 598-607.
• 16. Bass, A.D., Parce, J.W., Dechatelet, L.R., Szeja, P., Seeds, M.C. & Thomas, M. (1983) Flow cytometric studies of oxidative product formation by neutrophils: A graded response to membrane stimulation. J. Immunol. 130, 1910-1917.
• 17. Zielińska, M., Kostrzewa, A. & Ignatowicz, E. (2000) Antioxidative activity of flavonoids in stimulated human neutrophils. Folia Histochem. Cytobiol. 38, 25-30.
• 18. Ohkawa, H., Ohishi, N. & Yaga, K. (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95, 351-358.
• 19. Havsteen, B. (1983) Flavonoids, a class of natural products of high pharmacological potency. Biochem. Pharmacol. 32, 1141-1148.
• 20. Sugihara, N., Arakawa, T., Ohnishi, M. & Furuno, K. (1999) Anti- and pro-oxidative effects of flavonoids on metal-induced lipid hydroperoxide- dependent lipid peroxidation in cultured hepatocytes loaded with alpha-linolenic acid. Free Radic. Biol. Med. 27, 1313-1323.
• 21. Tauber, A.I., Fay, J.R. & Marletta, M.A. (1984) Flavonoid inhibition of the human neutrophil NADPH-oxidase. Biochem. Pharmacol. 33, 1367-1369.
• 22. Rice-Evans, C.A., Miller, N.J. & Paganga, G. (1996) Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic. Biol. Med. 20, 933-956.
• 23. Walgren, R.A, Lin, J.T, Kinne, R.K.H. & Walle, T. (2000) Cellular uptake of dietary flavonoid quercetin 4'-β-glucoside by sodium-dependent glucose transporter SGLT1. J. Pharmacol. Exp. Ther. 294, 837-843.
• 24. Walgren, R.A, Karnaky, K.J., Lindenmayer, G.E. & Walle, T. (2000) Efflux of dietary flavonoid quercetin 4'-β-glucoside across human intestinal Caco-2 cell monolayers by apical multidrug resistance-associated protein-2. J. Pharmacol. Exp. Ther. 294, 830-836.
• 25. Robak, J. & Gryglewski, R.J. (1996) Bioactivity of flavonoids. Pol. J. Pharmacol. 48, 555-564.
• 26. Robak, J. & Gryglewski, R.J. (1988) Flavonoids are scavengers of superoxide anions. Biochem. Pharmacol. 37, 837-841.
• 27. Okamura, H., Mimura, A., Yakou, Y., Niwano, M. & Takahara, Y. (1993) Anioxidant activity of tannins and flavonoids in Eucalyptus rostrata. Phytochemistry 33, 557-561.
• 28. Ignatowicz, E. & Rybczynska, M. (1994) Some biochemical and pharmacological aspects of free radical-mediated tissue damage. Pol. J. Pharmacol. 46, 103-114.