Signal transduction in confluent C3H 10T1/2 cells. The role of focal adhesion kinase.

• Authors: Joanna Miłoszewska , Halina Trembacz , Przemysław Janik
• Languages of publication: EN

Abstracts

EN

The activities of extracellular signal-regulated kinases (ERK1/ERK2) is required for proliferation of several types of cells. The performed analysis showed stimulation of ERK's by fetal calf serum (FCS) or fibronectin in the C3H 10T1/2 cell cultures at logarithmic phase of growth. The ERKs activity was not stimulated in confluent cells. This could not be accounted for a partial down regulation of ERK since its level was stable in both types of cells regardless of their density and kind of stimulation. Searching for ERK upstream elements we studied the integrin receptor gene transcript by RT-PCR and focal adhesion kinase (FAK) by Western blotting and phosphorylation assays. It was found that FCS and fibronectin stimulated phosphorylating activity of FAK in the cells at the logarithmic phase of growth, but were inefficient in the confluent cells. RT-PCR showed the presence of α5 and β1 integrin transcripts, and p125FAK was at the same level regardless of the type of stimulation. These data indicate that the ability of FAK to be activated plays an important role in ERK regulation and, in consequence in proliferation and growth inhibition during confluence.

Keywords

EN

integrins; FAK; ERK; fibronectin; contact inhibition

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2001
• Volume: 48
• Issue: 1
• Pages: 175-181

Dates

published

2001

received

2000-08-28

revised

2000-10-17

accepted

2001-02-1

Contributors

author

Joanna Miłoszewska

• Department of Cell Biology, The Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, W.K. Roentgena 5, 02-781 Warszawa, Poland

author

Halina Trembacz

• Department of Cell Biology, The Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, W.K. Roentgena 5, 02-781 Warszawa, Poland

author

Przemysław Janik

• Department of Cell Biology, The Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, W.K. Roentgena 5, 02-781 Warszawa, Poland

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