The KRR1 gene encodes a protein required for 18S rRNA synthesis and 40S ribosomal subunit assembly in Saccharomyces cerevisiae.

• Authors: Robert Gromadka , Joanna Rytka
• Languages of publication: EN

Abstracts

EN

The newly discovered Saccharomyces cerevisiae gene KRR1 (YCL059c) encodes a protein essential for cell viability. Krr1p contains a motif of clustered basic amino acids highly conserved in the evolutionarly distant species from yeast to human. We demonstrate that Krr1p is localized in the nucleolus. The KRR1 gene is highly expressed in dividing cells and its expression ceases almost completely when cells enter the stationary phase. In vivo depletion of Krr1p leads to drastic reduction of 40S ribosomal subunits due to defective 18S rRNA synthesis. We propose that Krr1p is required for proper processing of pre-rRNA and the assembly of preribosomal 40S subunits.

Keywords

EN

ribosome; rRNA processing; yeast

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2000
• Volume: 47
• Issue: 4
• Pages: 993-1005

Dates

published

2000

received

2000-07-12

accepted

2000-09-8

Contributors

author

Robert Gromadka

• Institute of Biochemistry and Biophysics, Polish Academy of Science, Warszawa, Poland

author

Joanna Rytka

• Institute of Biochemistry and Biophysics, Polish Academy of Science, Warszawa, Poland

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