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2000 | 47 | 4 | 963-971
Article title

In vitro effects of ozone on human erythrocyte membranes: An EPR study

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EN
Abstracts
EN
The effects of ozone at different concentrations (10, 30, 45 g/m3) on fluidity and thermotropic properties of erythrocyte membranes were investigated by EPR using two spin probes: 5-doxylstearic acid (5-DSA) and 16-doxylstearic acid (16-DSA). The effect of ozone on the erythrocyte membrane fluidity was a dose-dependent process. The ozone at concentration of 10 g/m3 caused rigidization of the membrane while at concentration of 45 g/m3 increased fluidity both on the surface and in the deeper hydrocarbon region of the membrane. Temperature transitions close to the polar heads region (monitored by 5-DSA) were not sensitive to an increase in ozone concentration. In the case of 16-DSA, low temperature thermotropic transition (around 20°C) gradually decreased with the increase of ozone concentration. High temperature transition (around 40°C) significantly differed at the ozone concentration of 10 g/m3 and 45 g/m3, being higher and lower, respectively, as compared to untreated cells. For the ozone concentration of 45 g/m3 the disappearance of the low temperature break and the appearance of two breaks at 37°C and 16°C were observed.
Publisher

Year
Volume
47
Issue
4
Pages
963-971
Physical description
Dates
published
2000
revised
2000-10-24
accepted
2000-11-15
Contributors
  • Department of Biophysics, The Ludwik Rydygier University of Medical Sciences in Bydgoszcz, Bydgoszcz, Poland
  • Department of Biophysics, The Ludwik Rydygier University of Medical Sciences in Bydgoszcz, Bydgoszcz, Poland
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv47i4p963kz
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