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2000 | 47 | 4 | 901-911
Article title

Peroxidation of proteins and lipids in suspensions of liposomes, in blood serum, and in mouse myeloma cells.

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EN
Abstracts
EN
There is growing evidence that proteins are early targets of reactive oxygen species, and that the altered proteins can in turn damage other biomolecules. In this study, we measured the effects of proteins on the oxidation of liposome phospholipid membranes, and the formation of protein hydroperoxides in serum and in cultured cells exposed to radiation-generated hydroxyl free radicals. Lysozyme, which did not affect liposome stability, gave 50% protection when present at 0.3 mg/ml, and virtually completely prevented lipid oxidation at 10 mg/ml. When human blood serum was irradiated, lipids were oxidized only after the destruction of ascorbate. In contrast, peroxidation of proteins proceeded immediately. Protein hydroperoxides were also generated without a lag period in hybrid mouse myeloma cells, while at the same time no lipid peroxides formed. These results are consistent with the theory that, under physiological conditions, lipid membranes are likely to be effectively protected from randomly-generated hydroxyl radicals by proteins, and that protein peroxyl radicals and hydroperoxides may constitute an important hazard to biological systems under oxidative stress.
Publisher

Year
Volume
47
Issue
4
Pages
901-911
Physical description
Dates
published
2000
received
2000-08-27
accepted
2000-11-03
Contributors
  • Department of Biological Sciences, Macquarie University, Sydney, Australia
author
  • Department of Biological Sciences, Macquarie University, Sydney, Australia
author
  • Department of Biological Sciences, Macquarie University, Sydney, Australia
  • Department of Biological Sciences, Macquarie University, Sydney, Australia
References
  • 1. Chance, B., Sies, H. & Boveris, A. (1979) Hydroperoxide metabolism in mammalian organs. Physiol. Rev. 59, 527-605.
  • 2. Freeman, B.A. & Crapo, J.D. (1982) Biology of disease. Lab. Invest. 47, 412-426.
  • 3. Sies, H. (ed.) (1985) Oxidative Stress. Academic Press, London.
  • 4. Sies, H. (ed) (1991) Oxidative Stress; Oxidants and Antioxidants. Academic Press, London.
  • 5. Bartosz, G. (1995) Druga Twarz Tlenu. Wydawnictwo Naukowe PWN (in Polish).
  • 6. Halliwell, B. & Gutteridge, J.M.C. (1989) Free Radicals in Biology and Medicine, 2nd ed., Clarendon Press, Oxford.
  • 7. Halliwell, B. & Gutteridge, J.M.C. (1990) Role of free radicals and catalytic metal ions in disease: An overview. Methods Enzymol. 186, 1-85.
  • 8. Halliwell, B., Gutteridge, J.M.C. & Cross, C.E. (1992) Free radicals, antioxidants, and human disease: Where are we now? J. Lab. Clin. Med. 119, 598-620.
  • 9. Fridovich, I. (1989) Superoxide dismutases. An adaptation to a paramagnetic gas. J. Biol. Chem. 264, 7761-7764.
  • 10. Halliwell, B. (1995) The biological significance of oxygen-derived species; in Active Oxygens in Biochemistry (Valentine, J.S., Foote, C.S., Greenberg, A. & Liebman, J.L., eds.) pp. 313-335, Blackie Academic & Professional, London.
  • 11. Grootveld, M., Bell, J.D., Halliwell, B., Aruoma, O.I., Bomford, A. & Sadler, P.J. (1989) Non-transferrin-bound iron in plasma from patients with idiopathic hemochromatosis. Characterisation by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy. J. Biol. Chem. 264, 4417-4422.
  • 12. Von Sonntag, C. (1987) The Chemical Basis of Radiation Biology. Taylor & Francis, London.
  • 13. Von Sonntag, C. & Schuchmann, H.-P. (1994) Supression of free radical reactions in biological systems. Considerations based on competition kinetics. Methods Enzymol. 233, 47-56.
  • 14. Caraceni, P., DeMaria, N., Ryu, H.S., Colantoni, A., Roberts, L., Maidt, M.L., Pye, Q., Bernardi, M., Van Thiel, D.H. & Floyd, R.A. (1997) Proteins but not nucleic acids are molecular targets for free radical attack during reoxygenation of rat hepatocytes. Free Radic. Biol. Med. 23, 339-344.
  • 15. Gay, K.F. (1998) Vitamins E plus C and interacting conutrients required for optimal health. BioFactors 7, 113-174.
  • 16. Halliwell, B. & Gutteridge, J.M.C. (1990) The antioxidants of human extracellular fluids. Arch. Biochem. Biophys. 280, 1-8.
  • 17. Simpson, J.A., Narita, S., Gieseg, S., Gebicki, S., Gebicki, J.M. & Dean, R.T. (1992) Long- lived reactive species on free radical-damaged proteins. Biochem. J. 282, 621-624.
  • 18. Gebicki, S. & Gebicki, J.M. (1993) Formation of peroxides in amino acids and proteins exposed to oxygen free radicals. Biochem. J. 289, 743-749.
  • 19. O'Donnell, J.H. & Sangster, D.F. (1970) Principles of Radiation Chemistry. Edward Arnold, London.
  • 20. Gay, C., Collins, J. & Gebicki, J.M. (1999) Determination of iron in solutions with the ferric-xylenol orange complex. Anal. Biochem. 273, 143-148.
  • 21. Gay, C., Collins, J. & Gebicki, J.M. (1999) Hydroperoxide assay with the ferric-xylenol orange complex. Anal. Biochem. 273, 149- 155.
  • 22. Levine, M., Wang, Y. & Rumsey, F.C. (1999) Analysis of ascorbic acid and dehydroascorbic acid in biological samples. Methods Enzymol. 299, 65-76.
  • 23. Frei, B., Stocker, R. & Ames, B.N. (1988) Antioxidant defenses and lipid peroxidation in human blood plasma. Proc. Natl. Acad. Sci. U.S.A. 85, 9748-9752.
  • 24. Frei, B., England, L. & Ames, B.N. (1989) Ascorbate is an outstanding antioxidant in human blood plasma. Proc. Natl. Acad. Sci. U.S.A. 86, 6377-6381.
  • 25. Gieseg, S., Duggan, S. & Gebicki, J.M. (2000) Peroxidation of proteins before lipids in U937 cells exposed to peroxyl radicals. Biochem. J. 350, 215-218.
  • 26. Gutteridge, J.M.C. & Quinlan, G.J. (1993) Antioxidant protection against organic and inorganic oxygen radicals by human blood plasma; The important primary role for iron-binding iron-oxidising proteins. Biochim. Biophys. Acta 1156, 144-150.
  • 27. Gebicki, J.M. (1997) Protein hydroperoxides as new reactive oxygen species. Redox Rep. 3, 99-110.
  • 28. Fu, S., Davies, M.J., Stocker, R. & Dean, R.T. (1998) Evidence for roles of radicals in protein oxidation in advanced human atherosclerotic plaque. Biochem. J. 333, 519-525.
  • 29. Clement, J.R., Armstrong, D.A., Klassen, N.V. & Gillis, H.A. (1972) Pulse radiolysis of aqueous papain. Can. J. Chem. 50, 2833-2840.
  • 30. Klapper, M.H. & Farraggi, M. (1979) Applications of pulse radiolysis to protein chemistry. Quart. Rev. Biophys. 12, 465-519.
  • 31. Lentner, C. (ed.) Geigy Scientific Tables (1984) pp. 101-140, vol. 3, Ciba-Geigy Ltd., Basle.
Document Type
Publication order reference
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YADDA identifier
bwmeta1.element.bwnjournal-article-abpv47i4p901kz
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