The response of L5178Y lymphoma sublines to oxidative stress: Antioxidant defence, iron content and nuclear translocation of the p65 subunit of NF-κB.

• Authors: Elżbieta Boużyk , Iwona Grądzka , Teresa Iwaneńko , Marcin Kruszewski , Barbara Sochanowicz , Irena Szumiel
• Languages of publication: EN

Abstracts

EN

We examined the response to hydrogen peroxide of two L5178Y (LY) sublines which are inversely cross-sensitive to hydrogen peroxide and X-rays: LY-R cells are radioresistant and hydrogen peroxide-sensitive, whereas LY-S cells are radiosensitive and hydrogen peroxide-resistant. Higher initial DNA breaks and higher iron content (potentially active in the Fenton reaction) were found in the hydrogen peroxide sensitive LY-R cells than in the hydrogen peroxide resistant LY-S cells, whereas the antioxidant defence of LY-R cells was weaker. In particular, catalase activity is twofold higher in LY-S than in LY-R cells. The content of monobromobimane-reactive thiols is 54% higher in LY-S than in LY-R cells. In contrast, the activity of glutathione peroxidase (GPx) is about two times higher in LY-R than in LY-S cells; however, upon induction with selenium the activity increases 15.6-fold in LY-R cells and 50.3-fold in LY-S cells. Altogether, the sensitivity difference is related to the iron content, the amount of the initial DNA damage, as well as to the efficiency of the antioxidant defence system. Differential nuclear translocation of p65-NF-κB in LY sublines is due to the more efficient antioxidant defence in LY-S than in LY-R cells.

Keywords

EN

iron; L5178Y murine lymphoma sublines; NF-κB; lovastatin; sensitivity to hydrogen peroxide; antioxidant defence; oxidative stress

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2000
• Volume: 47
• Issue: 4
• Pages: 881-888

Dates

published

2000

received

2000-08-02

revised

2000-09-26

accepted

2000-10-17

Contributors

author

Elżbieta Boużyk

• Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195 Warszawa, Poland

author

Iwona Grądzka

• Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195 Warszawa, Poland

author

Teresa Iwaneńko

• Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195 Warszawa, Poland

author

Marcin Kruszewski

• Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195 Warszawa, Poland

author

Barbara Sochanowicz

• Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195 Warszawa, Poland

author

Irena Szumiel

• Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195 Warszawa, Poland

References

• Alexander, P. & Mikulski, B. (1961) Mouse lymphoma cells with different radiosensitivities. Nature 192, 572-573.
• Baldwin, A.S., Jr. (1996) The NF-κB and IκB proteins: New discoveries and insights. Annu. Rev. Immunol. 14, 649-681.
• Beer, J.Z., Budzicka, E., Niepokojczycka, E., Rosiek, O., Szumiel, I. & Walicka, M. (1983) Loss of tumorigenicity with simultaneous changes in radiosensitivity and photosensitivity during in vitro growth of L5178Y murine lymphoma cells. Cancer Res. 43, 4736-4742.
• Boużyk, E., Buraczewska, I., Rosiek, O., Sochanowicz, B. & Szumiel, I. (1991) Hydrogen peroxide induced reproductive and interphase death in two strains of L5178Y murine lymphoma differing in radiation sensitivity. Radiat. Environ. Biophys. 30, 105-116.
• Boużyk, E., Iwaneńko, T., Jarocewicz, N., Kruszewski, M., Sochanowicz, B. & Szumiel, I. (1997) Antioxidant defence system in differentially hydrogen peroxide sensitive L5178Y sublines. Free Radic. Biol. Med. 22, 697- 704.
• Grądzka, I., Skierski, J. & Szumiel, I. (1998) DNA repair, cell cycle progression and cell death following camptothecin treatment in two murine lymphoma L5178Y sublines. Cell Biochem. Funct. 16, 239-252.
• Jaworska, A., Rosiek, O. & Witkowska, K. (1987) Activities of superoxide dismutase and catalase in two L5178Y murine lymphoma cell strains with different radiosensitivities. Nukleonika 32, 193-201.
• Kruszewski, M. & Szumiel, I. (1993) Cytotoxic and mutagenic effects of hydrogen peroxide in murine L5178Y sublines. Acta Biochim. Polon. 40, 42-45.
• Kruszewski, M. & Szumiel, I. (1994) DNA damage induced by hydrogen peroxide treatment at 4°C and 37°C in murine lymphoma L5178Y sublines. Acta Biochim. Polon. 41, 120-121.
• Kruszewski, M., Green, M.H., Lowe, J.E. & Szumiel, I. (1994) DNA strand breakage, cytotoxicity and mutagenicity of hydrogen peroxide treatment at 4deg;C and 37deg;C in L5178Y sublines. Mutat. Res. 308, 233-241.
• Kruszewski, M., Green, M.H., Lowe, J.E. & Szumiel, I. (1995) Comparison of effects of iron and calcium chelators on the response of L5178Y sublines to X-rays and H2O2. Mutat. Res. 326, 155-163.
• Kruszewski, M., Wojewódzka, M., Iwaneńko, T., Szumiel, I. & Okuyama, A. (1998) Differential inhibitory effect of OK-1035 on DNA repair in L5178Y murine lymphoma sublines with functional or defective repair of double strand breaks. Mutat. Res. 409, 31-36.
• Lipiński, P., Drapier, J.-C., Oliveira, L., Retmańska, H., Sochanowicz, B. & Kruszewski, M. (2000) Intracellular iron status as a hallmark of mammalian cells' susceptibility to oxidative stress: A study on L5178Y mouse lymphoma cell lines differentially sensitive to H2O2. Blood 95, 2960-2966.
• May, M.J. & Ghosh, S. (1997) Rel/NF-κB and IκB proteins: An overview. Semin. Cancer Biol. 8, 63-73.
• Mohan, N. & Meltz, M.-L. (1994) Induction of the nuclear factor κB after low-dose ionizing radiation involves a reactive oxygen intermediate signaling pathway. Radiat. Res. 140, 97-104.
• Santillo, M., Mondola, P., Gioielli Seru, R., Iossa, S., Annella, T., Vitale, M. & Bifulco, M. (1996) Inhibitors of Ras farnesylation revert the increased resistance to oxidative stress in K-ras transformed NIH 3T3 cells. Biochem. Biophys. Res. Commun. 229, 739-745.
• Schreck, R. & Bauerle, P.-A. (1991) A role for oxygen radicals as second messengers. Trends Cell Biol. 1, 39-42.
• Sinensky, M., Beck, L.A., Leonard, S. & Evans, R. (1990) Differential inhibitory effect of lovastatin on protein isoprenylation and sterol synthesis. J. Biol. Chem. 265, 19937-19941.
• Sochanowicz, B., Szumiel, I. & Grądzka, I. (1999) Nuclear translocation of the p65 subunit of NF-κ6B in L5178Y sublines differing in antioxidant defense. Radiat. Environ. Biophys. 38, 125-131.
• Storer, R.D., Kranyak, A.R., McKelvey, T.W., Elia, H.C., Goodrow, T.L. & Deluca, J.G. (1997) The mouse lymphoma L5178Y TK+/- cell line is heterozygous for a codon 170 mutation in the p53 tumor suppressor gene. Mutat. Res. 373, 157-165.
• Szumiel, I., Kapiszewska, M., Kruszewski, M., Iwaneńko, T. & Lange, C.S. (1995) Content of iron and copper in the nuclei and induction of pH 9-labile lesions in L5178Y sublines inversely cross-sensitive to H2O2 and x-rays. Radiat. Environ. Biophys. 34, 113-119.
• Wlodek, D. & Hittelman, W.N. (1988a) The relationship of DNA and chromosome damage to survival of synchronized X-irradiated L5178Y cells. I. Initial damage. Radiat. Res. 115, 550-565.
• Wlodek, D. & Hittelman, W.N. (1988b) The relationship of DNA and chromosome damage to survival of synchronized X-irradiated L5178Y cells. II. Repair. Radiat. Res. 115, 566-575.