Multidrug resistance-associated protein - reduction of expression in human leukaemia cells by antisense phosphorothioate olignucleotides.

• Authors: Wojciech Niewiarowski , Edyta Gendaszewska , Grzegorz Rębowski , Marzena Wójcik , Barbara Mikołajczyk , Mirosław Soszyński , Grzegorz Bartosz
• Languages of publication: EN

Abstracts

EN

Multidrug resistance-associated protein (MRP1) causes cellular drug resistance in several cancer cell lines. In this paper we show that antisense oligonucleotides decrease MRP1 expression in human leukaemia cells. We investigated biological activity of a series of 12 linear phosphorothioate oligonucleotides, complementary to several regions of MRP1 mRNA. The oligonucleotides were administered to leukaemia HL60/ADR cells overexpressing MRP1 protein. Then, the level of MRP1 mRNA was determined by means of semiquantitative RT-PCR and the protein level by reaction with specific monoclonal antibodies. Some of the investigated antisense oligonucleotides decrease the expression level of the MRP1 protein by 46% and its mRNA level by 76%.

Keywords

EN

antisense oligonucleotides; MRP; multidrug resistance

• Journal: Acta Biochimica Polonica
• Year: 2000
• Volume: 47
• Issue: 4
• Pages: 1183-1188

Dates

published

2000

received

2000-04-20

revised

2000-06-14

accepted

2000-09-20

Contributors

author

Wojciech Niewiarowski

• Polish Academy of Sciences, Centre of Molecular and Macromolecular Studies, Department of Bioorganic Chemistry, Łódź, Poland

author

Edyta Gendaszewska

• Polish Academy of Sciences, Centre of Molecular and Macromolecular Studies, Department of Bioorganic Chemistry, Łódź, Poland

author

Grzegorz Rębowski

• Polish Academy of Sciences, Centre of Molecular and Macromolecular Studies, Department of Bioorganic Chemistry, Łódź, Poland

author

Marzena Wójcik

• Polish Academy of Sciences, Centre of Molecular and Macromolecular Studies, Department of Bioorganic Chemistry, Łódź, Poland

author

Barbara Mikołajczyk

• Polish Academy of Sciences, Centre of Molecular and Macromolecular Studies, Department of Bioorganic Chemistry, Łódź, Poland

author

Mirosław Soszyński

• University of Łódź, Department of Molecular Biophysics, Łódź, Poland

author

Grzegorz Bartosz

• University of Łódź, Department of Molecular Biophysics, Łódź, Poland

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