Expression of β1-integrins and N-cadherin in bladder cancer and melanoma cell lines.

• Authors: Piotr Laidler , Dorota Gil , Anna Pituch-Noworolska , Dorota Ciołczyk , Dorota Książek , Małgorzata Przybyło , Anna Lityńska
• Languages of publication: EN

Abstracts

EN

Changes in the expression of integrins and cadherins might contribute to the progression, invasion and metastasis of transitional cell cancer of the bladder and of melanomas. The expression of α5 (P < 0.001), α2 and β1 (P < 0.05 - P < 0.001) integrin subunits in melanoma cells from noncutaneous metastatic sites (WM9, A375) were significantly increased as compared to cutaneous primary tumor (WM35) and metastatic (WM239) cell lines. These differences might be ascribed to the invasive character of melanoma cells and their metastasis to the noncutaneous locations. The significantly heterogeneous expression of β1 integrin subunit in two malignant bladder cancer cell lines (T24 and Hu456) and nonsignificant differences in the expression of α2, α3, and α5 subunits between malignant and non-malignant human bladder cell lines do not allow an unanimous conclusion on the role of these intergrin subunits in the progression of transitional cancer of bladder. The adhesion molecule, expressed in all studied melanoma and bladder cell lines, that reacted with anti-Pan cadherin monoclonal antibodies was identified as N-cadherin except in the HCV29 non-malignant ureter cell line. However, neither this nor any other bladder or melanoma cell line expressed E-cadherin. The obtained results imply that the replacement of E-cadherin by N-cadherin accompanied by a simultaneous increase in expression of a2, a3 and a5 integrin subunits clearly indicates an increase of invasiveness of melanoma and, to a lesser extent, of transitional cell cancer of bladder. High expression of N-cadherin and a5 integrin subunit seems to be associated with the most invasive melanoma phenotype.

Keywords

EN

cytofluorimetry; cancer; integrins; cadherins; cell lines

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2000
• Volume: 47
• Issue: 4
• Pages: 1159-1170

Dates

published

2000

received

2000-05-29

revised

2000-10-20

accepted

2000-11-14

Contributors

author

Piotr Laidler

• Institute of Medical Biochemistry, Collegium Medicum, Jagiellonian University, Kraków, Poland

author

Dorota Gil

• Institute of Medical Biochemistry, Collegium Medicum, Jagiellonian University, Kraków, Poland

author

Anna Pituch-Noworolska

• Department of Clinical Immunology, Polish-American Children's Hospital, Collegium Medicum, Jagiellonian University, Kraków, Poland

author

Dorota Ciołczyk

• Institute of Medical Biochemistry, Collegium Medicum, Jagiellonian University, Kraków, Poland

author

Dorota Książek

• Institute of Medical Biochemistry, Collegium Medicum, Jagiellonian University, Kraków, Poland

author

Małgorzata Przybyło

• Institute of Zoology, Department of Animal Physiology, Jagiellonian University, Kraków, Poland

author

Anna Lityńska

• Institute of Zoology, Department of Animal Physiology, Jagiellonian University, Kraków, Poland

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