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2000 | 47 | 4 | 1159-1170
Article title

Expression of β1-integrins and N-cadherin in bladder cancer and melanoma cell lines.

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EN
Abstracts
EN
Changes in the expression of integrins and cadherins might contribute to the progression, invasion and metastasis of transitional cell cancer of the bladder and of melanomas. The expression of α5 (P < 0.001), α2 and β1 (P < 0.05 - P < 0.001) integrin subunits in melanoma cells from noncutaneous metastatic sites (WM9, A375) were significantly increased as compared to cutaneous primary tumor (WM35) and metastatic (WM239) cell lines. These differences might be ascribed to the invasive character of melanoma cells and their metastasis to the noncutaneous locations. The significantly heterogeneous expression of β1 integrin subunit in two malignant bladder cancer cell lines (T24 and Hu456) and nonsignificant differences in the expression of α2, α3, and α5 subunits between malignant and non-malignant human bladder cell lines do not allow an unanimous conclusion on the role of these intergrin subunits in the progression of transitional cancer of bladder. The adhesion molecule, expressed in all studied melanoma and bladder cell lines, that reacted with anti-Pan cadherin monoclonal antibodies was identified as N-cadherin except in the HCV29 non-malignant ureter cell line. However, neither this nor any other bladder or melanoma cell line expressed E-cadherin. The obtained results imply that the replacement of E-cadherin by N-cadherin accompanied by a simultaneous increase in expression of a2, a3 and a5 integrin subunits clearly indicates an increase of invasiveness of melanoma and, to a lesser extent, of transitional cell cancer of bladder. High expression of N-cadherin and a5 integrin subunit seems to be associated with the most invasive melanoma phenotype.
Keywords
Publisher

Year
Volume
47
Issue
4
Pages
1159-1170
Physical description
Dates
published
2000
received
2000-05-29
revised
2000-10-20
accepted
2000-11-14
Contributors
author
  • Institute of Medical Biochemistry, Collegium Medicum, Jagiellonian University, Kraków, Poland
author
  • Institute of Medical Biochemistry, Collegium Medicum, Jagiellonian University, Kraków, Poland
  • Department of Clinical Immunology, Polish-American Children's Hospital, Collegium Medicum, Jagiellonian University, Kraków, Poland
  • Institute of Medical Biochemistry, Collegium Medicum, Jagiellonian University, Kraków, Poland
  • Institute of Medical Biochemistry, Collegium Medicum, Jagiellonian University, Kraków, Poland
  • Institute of Zoology, Department of Animal Physiology, Jagiellonian University, Kraków, Poland
  • Institute of Zoology, Department of Animal Physiology, Jagiellonian University, Kraków, Poland
References
  • Ashton-Key, M., Cowley, G.P. & Smith, M.E.F. (1995) Cadherins in reactive lymph nodes and lymphomas: High expression in anaplastic large cell lymphomas. Histopatology 28, 55-59.
  • Birchmeier, W. (1995) E-Cadherin as a tumor invasion suppressor gene. BioEssays 17, 97-99.
  • Bubenick, J., Baresova, M., Viklicky, V., Jakoubkova, J., Sainerova, H. & Donner, J. (1973) Established cell line of urinary bladder carcinoma (T24) containing tumor specific antigen. Int. J. Cancer 11, 765-773.
  • Caveda, L.I., Martin-Padura, P., Navarro, F., Brevario, M., Corado, D., Gulino, M. & Lampugnani Dejana, E. (1996) Inhibition of cultured cell growth by vascular endothelial cadherin (cadherin-5/VE-cadherin). J. Clin. Invest. 98, 886-893.
  • Christofori, G. & Semb, H. (1999) The role of the cell-adhesion molecule E-cadherin as a tumour-suppressor gene. Trends Biochem. Sci. 24, 73-76.
  • Danen, E.H., de Vries, T.J., Morandini, R., Ghanem, G.G., Ruiter, D.J. & van Muijen, G.N. (1996) E-Cadherin expression in human melanoma. Melanoma Res. 6, 127-131.
  • Fujita, S., Suzuki, H., Kinoshita, M. & Hirohashi, S. (1992) Inhibition of cell attachment, invasion and metastasis of human carcinoma cells by anti-integrin β1 subunit antibody. Jpn. J. Cancer Res. 83, 1317-1326.
  • Fukushima, Y., Ohnishi, T., Arita, N., Hayakawa, T. & Sekiguchi, K. (1998) Integrin α5β1-mediated interaction with laminin-5 stimulates adhesion, migration and invasion of malignant glioma cells. Int. J. Cancer 76, 63-72.
  • Furukawa, F., Fujii, K., Horiguchi, Y., Matsuyoshi, N., Fujita, M., Toda, K., Imamura, S., Wakita, H., Shirahama, S. & Tagikawa, M. (1997) Roles of E- and P-cadherin in the human skin. Microsc. Res. Tech. 38, 343-352.
  • Giard, D.J., Aaronson, S.A., Todaro, G.J., Arnstein, P., Kersey, J. H., Dosik, H. & Parks, W.P. (1973) In vitro cultivation of human tumors: Establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51, 1417-1423.
  • Giroldi, L.A., Bringuier, P.P., Shimazui, T., Jansen, K. & Schalken, J.A. (1999) Changes in cadherin-catenin complexes in the progression of human bladder carcinoma. Int. J. Cancer 82, 70-76.
  • Hazan, R.B., Kang, L., Whooley, B.P. & Borgen, P.I. (1997) N-Cadherin promotes adhesion between invasive breast cancer cells and the stroma. Cell Adhes Commun. 4, 399-411.
  • Hughes, P.E., Renshaw, M.W., Pfaff, M., Forsyth, J., Keivens, V.M., Schwartz, M.A. & Ginsberg, M.H. (1997) Supression of integrin activation: A novel function of a Ras/Raf-initiated MAP kinase pathway. Cell 88, 521-530.
  • Humphries, M.J. & Newham, P. (1998) The structure of cell adhesion molecules. Trends Cell Biol. 8, 78-83.
  • Huttenlocher, A., Lakonishok, M., Kinder, M., Wu, S. & Truong, T. (1998) Integrin and cadherin synergy regulates contact inhibition of migration and motile activity. J. Cell Biol. 141, 515-526.
  • Hsu, M.J., Wheelock, M.J., Jonson, K.R. & Herlyn, M. (1996) Shifts in cadherin profiles between human normal melanocytes and melanomas. J. Investig Dermatol Symp. Proc.. 1, 188-194.
  • Imao, T., Koshida, K., Endo, Y., Uchibayashi, T., Sasaki, T. & Namiki, M. (1999) Dominant role of E-cadherin in the progression of bladder cancer. J. Urol. 161, 692-698.
  • Judware, R. & Culp, L.A. (1997) Extracellular matrix and matrix receptors: Alterations during tumor progression. Encyclopedia of Cancer 1, 660-679.
  • Kawamura-Kodama, K., Tsutsui, J., Suzuki, S.T., Kanzaki, T. & Ozawa, M. (1998) N-Cadherin expressed on malignant T cell lymphoma cells is functional, and promotes heterotypic adhesion between the lymphoma cells and mesenchymal cells expressing N-cadherin. J. Invest. Dermatol. 112, 62-66.
  • Keely, P., Parise, L. & Juliano, R. (1998) Integrins and GTPases in tumour cell growth, motility and invasion. Trends Cell Biol. 8, 101-106.
  • Laemmli, U.K. (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227, 680-685.
  • Larue, L.C., Antos, S., Butz, O., Huber, V., Delmas, M. & Kemler, R. (1996) A role for cadherins in tissue formation. Development (Camb) 122, 3185-3194.
  • Liebert, M., Washington, R., Stein, J., Wedemeyer, G. & Grossman, H.B. (1994) Expression of the VLA b1 integrin family in bladder cancer. Am. J. Pathol. 144, 1016-1022.
  • Longhurst, C.M. & Jennings, L.K. (1998) Integrin- mediated signal transduction. Cell. Mol. Life Sci. 54, 514-526.
  • Marshall, J.F., Rutherford, D.C., Happerfield, L., Hanby, A., McCartney, A.C.E., Newton- Bishop, J. & Hart, I.R. (1998) Comparative analysis of integrins in vitro and in vivo in uveal and cutaneous melanomas. Br. J. Cancer 77, 522-529.
  • Matsuyoshi, N., Tanaka, T., Toda, K. & Imamura, S. (1997) Identification of novel cadherins expressed in human melanoma cells. J. Invest. Dermatol. 108, 908-913.
  • Melchiori, A., Mortarini, R., Carlone, S., Marchisio, P.C., Anichini, A., Noonan, D.N. & Albini, A. (1995) The α3β1 integrin is involved in melanoma cell migration and invasion. Exp. Cell Res. 219, 233-242.
  • Mialhe, A., Levacher, G., Champelovier, P., Martel, V., Serres, M., Knudsen, K. & Seigneurin, D. (2000) Expression of E-, P-, N-cadherins and catenins in human bladder carcinoma cell lines. J. Urol. 164, 826-835.
  • Monier-Gavelle, F. & Duband, J.-L. (1995) Control of N-cadherin-mediated intercellular adhesion in migrating neural crest cells in vitro. J. Cell Sci. 108, 3839-3853.
  • Mortarini, R., Gismondi, A., Santoni, A., Parmiani, G. & Anchini, A. (1993) Role of the α5β1 integrin receptor in the proliferative response to quiescent human melanoma cells to fibronectin. Cancer Res. 52, 4499-4506.
  • Natali, P.G., Nicotra, M.R., Filippo, F.Di. & Bigotti, A. (1995) Expression of fibronectin, fibronectin isoforms and integrin receptors in melanocytic lesions. Br. J. Cancer 71, 1243- 1247.
  • Pederson, L. (1977) A simplification of the protein assay method of Lowry which is more generally applicable. Anal. Biochem. 83, 346-356.
  • Saito, T., Kimura, M., Kawasaki, T., Sato, S. & Tomita, Y. (1996) Correlation between integrin α5 expression and the malignant phenotype of transitional cell carcinoma. British J. Cancer 73, 327-331.
  • Sanders, R., Mainiero, F. & Giancotti, F. (1998) The role of integrins in tumorigenesis and metastasis. Cancer Invest. 16, 329-344.
  • Schadendorf, D., Fichtner, I., Makki, A., Alijagic, S., Kupper, M., Mrowietz, U. & Henz, B.M. (1996) Metastatic potential of human melanoma cells in nude mice characterisation of phenotype, cytokine secretion and tumour-associated antigens. Br. J. Cancer 74, 194- 199.
  • Schofield, K., D'Aquila, T. & Rimm, D.L. (1997) The cell adhesion molecule, E-cadherin, distinguishes mesothelial cells from carcinoma cells in fluids. Cancer. 81, 293-298.
  • Shin, E.-Y., Lee, J.-Y., Park, M.-K., Jeong, G.-B., Kim, E.-G. & Kim, S.-Y. (1999) H-Ras is a negative regulator of α3β1 integrin expression in ECV304 endothelial cells. Biochem. Biophys. Res. Commun. 257, 95-99.
  • Takeichi, M. (1991) Cadherin cell adhesion receptors as a morphogenetic regulator. Science 251, 1451-1455.
  • Tawil, N.J., Gowri, V., Dioneidi, M., Nip, J., Carbonetto, S. & Brodt, P. (1996) Integrin α3β1 can promote adhesion and spreading of metastatic breast carcinoma cells on the lymph node stroma. Int. J. Cancer. 66, 703-710.
  • Tran, N.L., Nagle, R.B., Cress, A.E. & Heimark, R.L. (1999) N-Cadherin expression in human prostate carcinoma cell lines. An epithelial- mesenchymal transformation mediating adhesion with stromal cells. Am. J. Pathol. 155, 787-798.
  • Vilien, M., Christensen, B., Wolf, H., Rassmussen, F., Hou-Jensen, C. & Povlsen, C.O. (1983) Comparative studies of normal, spontaneously' transformed and malignant human urothelium cells in vitro. Eur. J. Cancer Clin. Oncol. 19, 775-789.
  • Voura, E.B., Sandig, M. & Siu, C.H. (1998) Cell-cell interactions during transendothelial migration of tumor cells. Microsc. Res. Tech. 43, 265-275.
  • Wakatsuki, S., Watanabe, R., Saito, K., Saito, T., Katagiri, A., Sato, S. & Tomita, Y. (1996) Loss of human E-cadherin (ECD) correlated with invasiveness of transitional cell cancer in the renal pelvis, ureter and urinary bladder. Cancer Lett. 103, 11-17.
  • Wu, Ch., Keightley, S., Leung-Hagestein, Ch., Radeval, G., Coppolino, M., Goicoechea, S., McDonald, A.J. & Dedhar, S. (1998) Integrin- linked protein kinase regulates fibronectin matrix assembly, E-cadherin expression, and tumorigenicity. J. Biol. Chem. 273, 528-536.
  • Zutter, M.M., Sun, H. & Santoro, S. (1998) Altered integrin expression and the malignant phenotype: The contribution of multiple integrated integrin receptors. J. Mammary Gland Biol. Neoplasia. 3, 191-199.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv47i4p1159kz
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