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2000 | 47 | 4 | 1037-1044
Article title

The DFF40/CAD endonuclease and its role in apoptosis.

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EN
Abstracts
EN
The sequential generation of large-scale DNA fragments followed by internucleosomal chromatin fragmentation is a biochemical hallmark of apoptosis. One of the nucleases primarily responsible for genomic DNA fragmentation during apoptosis is called DNA Fragmentation Factor 40 (DFF40) or Caspase-activated DNase (CAD). DFF40/CAD is a magnesium-dependent endonuclease specific for double stranded DNA that generates double strand breaks with 3'-hydroxyl ends. DFF40/CAD is activated by caspase-3 that cuts the nuclease's inhibitor DFF45/ICAD. The nuclease preferentially attacks chromatin in the internucleosomal linker DNA. However, the nuclease hypersensitive sites can be detected and DFF40/CAD is potentially involved in large-scale DNA fragmentation as well. DFF40/CAD-mediated DNA fragmentation triggers chromatin condensation that is another hallmark of apoptosis.
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Year
Volume
47
Issue
4
Pages
1037-1044
Physical description
Dates
published
2000
received
2000-08-30
revised
2000-11-10
accepted
2000-11-13
Contributors
author
  • Department of Experimental and Clinical Radiobiology, Center of Oncology, 44-100 Gliwice, Poland
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv47i4p1037kz
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