Effect of amyloid beta peptide on poly(ADP-ribose) polymerase activity in adult and aged rat hippocampus.

• Authors: Joanna B Strosznajder , Henryk Jęśko , Robert P Strosznajder
• Languages of publication: EN

Abstracts

EN

It is suggested that the fibrillar amyloid beta peptide (Aβ) in brain plays a direct role in neurodegeneration in Alzheimer's disease, probably through activation of reactive oxygen species formation. Free radicals and numerous neurotoxins elicit DNA damage that subsequently activates poly(ADP-ribose) polymerase (PARP, EC 2.4.2.30). In this study the effect of neurotoxic fragment (25-35) of full length Aβ peptide on PARP activity in adult and aged rat hippocampus was investigated. In adult (4 month old) rat hippocampus the Aβ 25-35 peptide significantly enhanced PARP activity by about 80% but had no effect on PARP activity in cerebral cortex and in hippocampus from aged (24-27 month old) rats. The effect of Aβ peptide was reduced by half by the nitric oxide synthase inhibitor N-nitro-L-arginine. Stimulation of glutamate receptor(s) itself enhanced PARP activity by about 80% in adult hippocampus. However, Aβ 25-35 did not exert any additional stimulatory effect. These results indicate that Aβ, through NO and probably other free radicals, induces activation of DNA bound PARP activity exclusively in adult but not in aged hippocampus.

Keywords

EN

aging; amyloid; poly(ADP-ribose) polymerase; hippocampus; neurotoxicity

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2000
• Volume: 47
• Issue: 3
• Pages: 847-854

Dates

published

2000

received

2000-03-09

accepted

2000-05-08

Contributors

author

Joanna B Strosznajder

• Department of Cellular Signalling, Medical Research Centre, Polish Academy of Sciences, A. Pawińskiego 5, 02-106 Warszawa, Poland

author

Henryk Jęśko

• Department of Cellular Signalling, Medical Research Centre, Polish Academy of Sciences, A. Pawińskiego 5, 02-106 Warszawa, Poland

author

Robert P Strosznajder

• Department of Neurophysiology, Medical Research Centre, Polish Academy of Sciences, A. Pawińskiego 5, 02-106 Warszawa, Poland

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