Partial deglycosylation of α subunit modifies sturgeon gonadotropin function.

• Authors: Henriks Zenkeviès , Vija Vose , Ilze Vosekalne , Ausma Bcena
• Languages of publication: EN

Abstracts

EN

Chemical deglycosylation (dg) of sturgeon Acipenser gueldenstaedti Br. (αGTH) resulted in the loss of 83% of its initial carbohydrate content. It altered also recombinant dgαGTH + βGTH dimer molecule, reducing its immunoreactivity by 30%, and fully blocking the hormonal function. CD spectroscopy showed that deglycosylation led to changes in the secondary structure of dgαGTH and in the α-β recombinant. The sugar moiety of sturgeon αGTH is suggested to play an important role in maintaining the biological function of the hormone dimer molecule.

Keywords

EN

α subunit; CD spectrum; sturgeon GTH; gonadotropic hormone; deglycosylation

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2000
• Volume: 47
• Issue: 3
• Pages: 815-819

Dates

published

2000

received

1999-11-24

revised

2000-04-26

accepted

2000-06-05

Contributors

author

Henriks Zenkeviès

• Laboratory of Biochemistry, Institute of Biology, Latvian University, Salaspils, Latvia

author

Vija Vose

• Laboratory of Biochemistry, Institute of Biology, Latvian University, Salaspils, Latvia

author

Ilze Vosekalne

• Laboratory of Peptide Chemistry, Latvian Institute of Organic Synthesis, Riga, Salaspils, Latvia

author

Ausma Bcena

• Laboratory of Biochemistry, Institute of Biology, Latvian University, Salaspils, Latvia

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