The effect of hexadecaprenol on molecular organisation and transport properties of model membranes.

• Authors: Teresa Janas , Krzysztof Nowotarski , Wiesław I Gruszecki , Tadeusz Janas
• Languages of publication: EN

Abstracts

EN

The Langmuir monolayer technique and voltammetric analysis were used to investigate the properties of model lipid membranes prepared from dioleoylphosphatidylcholine (DOPC), hexadecaprenol (C80), and their mixtures. Surface pressure- molecular area isotherms, current-voltage characteristics, and membrane conductance-temperature were measured. Molecular area isobars, specific molecular areas, excess free energy of mixing, collapse pressure and collapse area were determined for lipid monolayers. Membrane conductance, activation energy of ion migration across the membrane, and membrane permeability coefficient for chloride ions were determined for lipid bilayers. Hexadecaprenol decreases the activation energy and increases membrane conductance and membrane permeability coefficient. The results of monolayer and bilayer investigations show that some electrical, transport and packing properties of lipid membranes change under the influence of hexadecaprenol. The results indicate that hexadecaprenol modulates the molecular organisation of the membrane and that the specific molecular area of polyprenol molecules depends on the relative concentration of polyprenols in membranes. We suggest that hexadecaprenol modifies lipid membranes by the formation of fluid microdomains. The results also indicate that electrical transmembrane potential can accelerate the formation of pores in lipid bilayers modified by long chain polyprenols.

Keywords

EN

voltammetric studies.; bilayer lipid membranes; Langmuir film studies; long-chain polyprenol; lipid monolayer

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2000
• Volume: 47
• Issue: 3
• Pages: 661-673

Dates

published

2000

received

2000-07-26

accepted

2000-08-03

Contributors

author

Teresa Janas

• Biophysics Laboratory, Department of Physics, Technical University, Zielona Góra, Poland

author

Krzysztof Nowotarski

• Department of Biophysics, Institute of Biotechnology and Environmental Sciences, Monte Cassino 21B, 65-561 Zielona Góra, Poland

author

Wiesław I Gruszecki

• Department of Biophysics, Institute of Physics, Maria Curie-Skłodowska University, Lublin, Poland

author

Tadeusz Janas

• Department of Biophysics, Institute of Biotechnology and Environmental Sciences, Monte Cassino 21B, 65-561 Zielona Góra, Poland

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