Gemini (dimeric) surfactant perturbation of the human erythrocyte.

• Authors: Martina Dubnièková , Malgorzata Bobrowska-Hägerstrand , Thomas Söderström , Aleš Igliè , Henry Hägerstrand
• Languages of publication: EN

Abstracts

EN

We studied the ability of di-cationic gemini surfactantsdi (amphiphiles), i.e. 1,4-butanediammonium-N,N-dialkyl-N,N,N',N'-tetramethyl bromides (Di-Cm-di-QAS (s = 4), where m = 8,11,13,16 and s = the number of alkyl groups in the spacer) to induce shape alteration, vesiculation, haemolysis and phosphatidylserine exposure in human erythrocytes, and to protect erythrocytes against hypotonic haemolysis. At high sublytic concentrations the Di-Cm-di-QAS (s = 4) amphiphiles rapidly induced echinocytic (spiculated) shapes and a release of exovesicles, mainly in the form of tubes, from the cell surface. Following 60 min incubation erythrocytes were sphero-echinocytic and a few cells with invaginations/endovesicles were observed. No phosphatidylserine exposure was detected. The haemolytic potency increased with an increase of the alkyl chain length. At sublytic concentrations the Di-Cm-di-QAS (s = 4) amphiphiles protected erythrocytes against hypotonic haemolysis. It is suggested that the Di-Cm-di-QAS (s = 4) amphiphiles perturb the membrane in a similar way as single-chain cationic amphiphiles, but that they do not easily translocate to the inner membrane leaflet.

Keywords

EN

TEM; flow cytometry; protection against hypotonic haemolysis; exovesiculation; human erythrocyte; detergent; cationic amphiphile; shape alteration; haemolysis

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2000
• Volume: 47
• Issue: 3
• Pages: 651-660

Dates

published

2000

received

2000-07-18

accepted

2000-07-31

Contributors

author

Martina Dubnièková

• Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, Bratislava, Slovakia

author

Malgorzata Bobrowska-Hägerstrand

• Department of Biology, Åbo Akademi University, Biocity, Åbo/Turku, Finland

author

Thomas Söderström

• Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Biocity, Åbo/Turku, Finland

author

Aleš Igliè

• >Laboratory of Applied Physics, Faculty of Electrical Engineering, University of Ljubljana, Ljubljana, Slovenia

author

Henry Hägerstrand

• Department of Biology, Åbo Akademi University, Biocity, Åbo/Turku, Finland

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