Detection of damage-recognition proteins from human lymphocytes.

• Authors: Joanna Łanuszewska , Anna Cudak , Joanna Rzeszowska-Wolny , Piotr Widłak
• Languages of publication: EN

Abstracts

EN

Proteins recognizing and binding to damaged DNA (DDB-proteins) were analyzed in human lymphocytes obtained from healthy donors. Using an electrophoretic mobility shift assay several complexes between nuclear extract proteins and damaged DNA were detected: a complex specific for DNA damaged by N-acetoxy-N-acetylaminofluorene, another complex specific for UV-irradiated DNA, and two complexes specific for DNA damaged by cis-dichlorodiammine platinum. All the detected complexes differed in electrophoretic mobility and possibly contained different proteins. Complexes specific for free DNA ends were also detected in protein extracts from lymphocytes.

Keywords

EN

damage recognition; human lymphocytes; damaged DNA-binding proteins; repair capacity

• Journal: Acta Biochimica Polonica
• Year: 2000
• Volume: 47
• Issue: 2
• Pages: 443-450

Dates

published

2000

received

2000-01-17

accepted

2000-04-26

Contributors

author

Joanna Łanuszewska

• Department of Experimental and Clinical Radiobiology, Center of Oncology, 44-100 Gliwice, Poland

author

Anna Cudak

• Department of Experimental and Clinical Radiobiology, Center of Oncology, 44-100 Gliwice, Poland

author

Joanna Rzeszowska-Wolny

• Department of Experimental and Clinical Radiobiology, Center of Oncology, 44-100 Gliwice, Poland

author

Piotr Widłak

• Department of Experimental and Clinical Radiobiology, Center of Oncology, 44-100 Gliwice, Poland

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