The participation of ribosome-UDP-GalNAc complex in the initiation of protein glycosylation in vitro

• Authors: Anna Paszkiewicz-Gadek , Halina Porowska , Andrzej Gindzieński
• Languages of publication: EN

Abstracts

EN

The gastric epithelial cells ribosome-UDP-GalNAc complex is a donor of UDP- GalNAc as the substrate for N-acetylgalactosaminyltransferase, which catalyse the transfer of GalNAc residue to the polypeptide, existing on polysomes. It was observed that the deglycosylated porcine mucin and synthetic peptide (PTSSPIST) can be also glycosylated with participation of N-acetylgalactosaminyltransferase and ribosome- UDP-GalNAc complex. The probability of the ribosome-UDP-GalNAc complex as an intermediate in the O-glycosylation is considered.

Keywords

EN

glycosylation; UDP-GalNAc-transferase; UDP-GalNAc; apomucin; ribosome

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2000
• Volume: 47
• Issue: 2
• Pages: 421-426

Dates

published

2000

received

1999-08-12

revised

2000-03-23

accepted

2000-04-5

Contributors

author

Anna Paszkiewicz-Gadek

• Department of General and Organic Chemistry, Institute of Chemistry Medical Academy, 15-230 Białystok 8, Poland

author

Halina Porowska

• Department of General and Organic Chemistry, Institute of Chemistry Medical Academy, 15-230 Białystok 8, Poland

author

Andrzej Gindzieński

• Department of General and Organic Chemistry, Institute of Chemistry Medical Academy, 15-230 Białystok 8, Poland

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