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2000 | 47 | 2 | 385-391
Article title

Direct transfer of IL-12 gene into growing Renca tumors.

Content
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EN
Abstracts
EN
We investigated the feasibility of transferring naked plasmid DNA containing a therapeutic gene (IL-12) into mice harboring growing Renca tumors. We found that naked DNA transferred into growing Renca and B16(F10) tumors gives higher expression level of reporter gene than complexes of DNA with DDAB/ DOPE or DC-Chol/DOPE. Transfer of naked DNA carrying the IL-12 gene into growing Renca tumors causes a distinct therapeutic effect that depends on the time span between inoculation of mice with cancer cells and the beginning of the therapy. Therapy started on day 3 resulted in total cure (100%) of mice.
Keywords
Year
Volume
47
Issue
2
Pages
385-391
Physical description
Dates
published
2000
received
2000-03-9
References
  • 1. Anderson, W.F. (1998) Human gene therapy. Nature 392, 25-30.
  • 2. Lasic, D.D. & Templeton, N.S. (1996) Liposomes in gene therapy. Adv. Drug Deliv. Rev. 20, 221-266.
  • 3. Muramatsu, T., Nakamura, A. & Park, H.M. (1998) In vivo electroporation: A powerful and convenient means of nonviral gene transfer to tissues of living animals. Int. J. Mol. Med. 1, 55-62.
  • 4. Yang, N.S. & Sun, W.H. (1995) Gene gun and other non-viral approaches for cancer gene therapy. Nat Med. 1, 481-483.
  • 5. Wolff, J.A., Malone, R.W., Williams, P., Chong, W., Acsadi, G., Jani, A. & Felgner, P.L. (1990) Direct gene transfer into mouse muscle in vivo. Science 247, 1465-1468.
  • 6. Raz, E., Carson, D.A., Parker, S.E., Parr, T.B., Abai, A.M., Aichinger, G., Gromkowski, S.H., Singh, M., Lew, D., Yankauckas, M.A., Baird, S.M. & Rhodes, G.H. (1994) Intradermal gene immunization: The possible role of DNA uptake in the induction of cellular immunity to viruses. Proc. Natl. Acad. Sci. U.S.A. 91, 9519-9523.
  • 7. Hickman, M.A., Malone, R.W., Lehmann- Bruinsma, K., Sih, T.R., Knoell, D., Szoka, F.C., Walzem, R., Carlson, D.M. & Powell, J.S. (1994) Gene expression following direct injection of DNA into liver. Hum. Gene Ther. 5, 1477-1483.
  • 8. Yang, J.P. & Huang, L. (1996) Direct gene transfer to mouse melanoma by intratumor injection of free DNA. Gene Ther. 3, 542-548.
  • 9. Trinchieri, G. (1998) Interleukin-12: A cytokine at the interface of inflammation and immunity. Adv. Immun. 70, 83-243.
  • 10. Tan, J., Newton, C.A., Djeu, J.Y., Gutsch, D.E., Chang, A.E., Yang, N.S., Klein, T.W. & Yu, H. (1996) Injection of complementary DNA encoding interleukin-12 inhibits tumor establishment at a distant site in a murine renal carcinoma model. Cancer Res. 56, 3399-3403.
  • 11. Rakhmilevich, A.L., Turner, J., Ford, M.J., McCabe, D., Sun, W.H., Sondel, P.M., Grota, K. & Yang, N.S. (1996) Gene gun-mediated skin transfection with interleukin 12 gene results in regression of established primary and metastatic murine tumors. Proc. Natl. Acad. Sci. U.S.A. 93, 6291-6296.
  • 12. Rakhmilevich, A.L., Janssen, K., Turner, J., Culp, J. & Yang, N.S. (1997) Cytokine gene therapy of cancer using gene gun technology: Superior antitumor activity of interleukin-12. Hum. Gene Ther. 8, 1303-1311.
  • 13. Bramson, J.L., Hitt, M., Addison, C.L., Muller, W.J., Gauldie, J. & Graham, F.L. (1996) Direct intratumoral injection of an adenovirus expressing interleukin-12 induces regression and long-lasting immunity that is associated with highly localized expression of interleukin-12. Hum. Gene Ther. 7, 1995-2002.
  • 14. Caruso, M., Pham-Nguyen, K., Kwong, Y.L., Xu, B., Kosai, K.I., Finegold, M., Woo, S.L.C. & Chen, S.H. (1996) Adenovirus-mediated interleukin-12 gene therapy for metastatic colon carcinoma. Proc. Natl. Acad. Sci. U.S.A. 93, 11302-11306.
  • 15. Rakhmilevich, A.L., Timmins, J.G., Janssen, K., Pohlmann, E.L., Sheehy, M.J. & Yang, N.S. (1999) Gene gun-mediated IL-12 gene therapy induces antitumor effects in the absence of toxicity: A direct comparison with systemic IL-12 protein therapy. J. Immunother. 22, 135-144.
  • 16. Obana, S., Miyazawa, H., Hara, E., Tamura, T., Nariuchi, H., Takata, M., Fujimoto, S. & Yamamoto, H. (1995) Induction of anti-tumor immunity by mouse tumor cells transfected with mouse interleukin-12 gene. Jpn. J. Med. Sci. Biol. 48, 221-236.
  • 17. Hartikka, J., Sawdey, M., Cornefert-Jensen, F., Margalith, M., Barnhart, K., Nolasco, M., Vahlsing, H.L., Meek, J., Marquet, M., Hobart, P., Norman, J. & Manthorpe, M. (1996) An improved plasmid DNA expression vector for direct injection into skeletal muscle. Hum. Gene Ther. 7, 1205-1217.
  • 18. Wicks, I.P., Howell, M.L., Hancock, T., Kohsaka, H., Olee, T. & Carson, D.A. (1995) Bacterial lipopolysaccharide copurifies with plasmid DNA: Implications for animal models and human gene therapy. Hum. Gene Ther. 6, 317-323.
  • 19. Rose, J.K., Buonocore, L. & Whitt, M.A. (1991) A new cationic liposome reagent mediating nearly quantitative transfection of animal cells. BioTechniques 10, 520-525.
  • 20. Gao, X. & Huang, L. (1991) A novel cationic liposome reagent for efficient transfection of mammalian cells. Biochem. Biophys. Res. Commun. 179, 280-285.
  • 21. Bramson, J.L., Hitt, M., Addison, C.L., Muller, W.J., Gauldie, J. & Graham, F.L. (1996) Direct intratumoral injection of an adenovirus expressing interleukin-12 regression and long-lasting immunity that is associated with highly localized expression of interleukin-12. Hum. Gene Ther. 7, 1995-2002.
  • 22. Szala, S., Missol, E., Sochanik, A. & Strozyk, M. (1996) The use of cationic liposomes DC-CHOL/DOPE and DDAB/DOPE for direct transfer of Escherichia coli cytosine deaminase gene into growing melanoma tumors. Gene Ther. 3, 1026-1031.
  • 23. Colombo, M.P., Vagliani, M., Spreafico, F., Parenza, M., Chiodoni, C., Melani, C. & Stoppacciaro, A. (1996) Amount of interleukin 12 available at the tumor site is critical for tumor regression. Cancer Res. 56, 2531-2534.
  • 24. Coll, J.L., Negoescu, A., Louis, N., Sachs, L., Tenaud, C., Girardot, V., Demeinex, B., Brambilla, E., Brambilla, C. & Favrot, M. (1998) Antitumor activity of bax and p53 naked gene transfer in lung cancer: In vitro and in vivo analysis. Hum. Gene Ther. 9, 2063- 2074.
  • 25. Sato, Y., Roman, M., Tighe, H., Lee, D., Corr, M., Nguyen, M.D., Silverman, G.J., Lotz, M., Carson, D.A. & Raz, E. (1996) Immunostimulatory DNA sequences necessary for effective intradermal gene immunization. Science 273, 352-354.
  • 26. Moelling, K. (1998) Naked DNA the poor man's gene therapy? Gene Ther. 5, 573-574.
  • 27. Krieg, A.M. (1999) Direct immunologic activities of CpG DNA and implications for gene therapy. J. Gene Med. 1, 56-63.
Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv47i2p385kz
Identifiers
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