Preferences help
enabled [disable] Abstract
Number of results
2000 | 47 | 2 | 281-292
Article title

Antioxidant defense in centenarians (a preliminary study).

Title variants
Languages of publication
The study was designed to assess the antioxidant defense mechanisms, either enzymatic or non-enzymatic, in a group of sixteen centenarians (one male and fifteen female subjects aged 101 to 105 years) living in the Upper Silesia district (Poland) in order to evaluate the potential role of antioxidant defenses in human longevity. The results of our preliminary study showed that in comparison with young healthy female adults the centenarians had significantly higher red blood cell glutathione reductase and catalase activities and higher, although insignificantly, serum vitamin E level.
Physical description
  • Academy of Physical Education, Katowice, Poland
  • Geriatric Hospital, Katowice, Poland
  • Medical Academy, Poznań, Poland
  • Silesian Medical University, Katowice, Poland
  • Academy of Physical Education, Katowice, Poland
  • Silesian Medical University, Katowice, Poland
  • Aebi, H. (1974) Catalase; in: Methods of Enzymatic Analysis (Bergmayer, H.U., ed.) pp. 673-683.
  • Ames, B.N., Cathart, R., Schwiers, E. & Hochstein, P. (1981) Uric acid provides an antioxidant defense in humans against oxidant- and radical-caused aging and cancer: A hypothesis. Proc. Natl. Acad. Sci. U.S.A. 78, 6858-6862.
  • Behrens, W.A. & Madere, R. (1986) Alpha- and gamma-tocopherol concentrations in human serum. J. Am. Coll. Nutr. 5, 91-96.
  • Bierhaus, A., Ziegler, R. & Nawroth, P.P. (1998) Molecular mechanisms of diabetic angiopathy clues for innovative therapeutic interventions. Hormone Res. 50 (S1), 1-5.
  • Buege, J.A. & Aust, S.D. (1978) Microsomal lipid peroxidation. Methods Enzymol. 52, 302-310.
  • Casesnoves, A., Mauri, M., Dominguez, J.R., Alfayate, R. & Pico, A.M. (1998) Influence of anti-insulin antibodies on insulin immunoassays of the autoimmune insuline syndrome. Ann. Clin. Biochem. 35, 768-774.
  • Cao, G., Booth, S.L., Sadowski, J.A. & Prior, R.L. (1998) Increases in human plasma antioxidant capacity after consumption of controlled diets high in fruit and vegetables. Am. J .Clin. Nutr. 68, 1081-1087.
  • Caprari, P., Scuteri, A., Salvati, A.M., Bauco, C., Cantafora, A., Masella, R., Modesti, D., Tarzia, A. & Marigliano, V. (1999) Aging and red blood cell membrane: A study of centenarians. Exp. Gerontol. 34, 47-57.
  • Clarke, R., Woodhouse, P., Ulvik, A., Frost, C., Sherliker, P., Refsum, H., Ueland, P.M. & Khaw, K.T. (1998) Variability and determinants of total homocysteine concentrations in plasma in an elderly population. Clin. Chem. 44, 102-107.
  • Cobler, J.L., Akiyama, T. & Murphy, G.W. (1989) Permanent pacemakers in centenarians, J. Am. Geriatr. Soc. 37, 753-756.
  • Cutler, R.G. (1985) Peroxide-producing potential of tissues: Inverse correlation with longevity of mammalian species. Proc. Natl. Acad. Sci. U.S.A. 82, 4798-4802.
  • Danch, A. & Dróżdż, M. (1996) A simplified technique of fluorometric selenium assay in biological material. Diagn. Lab. 32, 529-534.
  • Elmadfa, I., Both-Bedenbender, N., Sierakowski, B. & Steinhagen-Thiessen, E. (1986) Significance of vitamin E in aging. Z. Gerontol. 19, 206-214.
  • Fielding, R.A. & Meydani, M. (1997) Exercise, free radical generation, and aging. Aging (Milano) 9, 12-18.
  • Flohe, L. & Gunzler, W.A. (1984) Assays of glutathione peroxidase. Methods Enzymol. 105, 114-120.
  • Franceschi, C., Monti, D., Scarfi, M.R., Zeni, O., Temperani, P., Emilia, G., Sansoni, P., Lioi, M.B., Troiano, L. & Agnesini, C. (1992) Genomic instability and aging. Studies in centenarians (successful aging) and in patients with Down's syndrome (accelerated aging). Ann. N.Y. Acad. Sci. 663, 4-16.
  • Glatzle, D., Körner, W.F., Christeller, S. & Wiss, O. (1970) Method for the detection of a biochemical riboflavin deficiency. Stimulation of NADPH2-dependent glutathione reductase from human erythrocytes by FAD in vitro. Investigations on the vitamin B2 status in healthy people and geriatric patients. Int. Z. Vitaminforsch. 40, 166-183.
  • Gottfries, C.G., Lehman, W. & Regland, B. (1998) Early diagnosis of cognitive impairment in the elderly with the focus on Alzheimer's disease. J. Neural. Transm. 105, 773-786.
  • Hallfrisch, J., Muller, D.C. & Singh, V.N. (1994) Vitamin A and E intakes, and plasma concentrations of retinol, beta-carotene, and alpha-tocopherol in men and women of the Baltimore Longitudinal Study of Aging. Am. J. Clin. Nutrit. 60, 176-182.
  • Harman, D. (1992) Free radical theory of aging. Mutat. Res. 275, 257-266.
  • JakubowskiGdańskZ., Kabata, J., Kalinowski, L., Szczepańska-Konkel, M. & Angielski, S. (1995) Laboratory analyses in everyday practice, reference values and their interpretation, MAKMed, p. 343, Gdańsk (in Polish).
  • Lombeck, I., Kasperek, K., Harbisch, H.D., Feinendegen, L.E. & Bremer, H.J. (1977) The selenium state of healthy children. I. Serum selenium concentrations at different ages; Activity of glutathione peroxidase of erythrocytes at different ages; Selenium content of food of infants. Eur. J. Pediatr. 125, 81-88.
  • Lopez-Torres, M., Perez-Campo, R., Rojas, C., Cadenas, S. & Barja, G. (1993) Simultaneous induction of SOD, glutathione reductase, GSH, and ascorbate in liver and kidney correlates with survival during aging. Free Radical Biol. Med. 15, 133-142.
  • Maulik, N., Yoshida, T. & Das, D.K. (1998) Oxidative stress developed during the reperfusion of ischemic myocardium induces apoptosis. Free Radical Biol. Med. 24, 869-875.
  • Morrissey, P.A. & Sheehy, P.J. (1999) Optimal nutrition: Vitamin E. Proc. Nutr. Soc. 58, 459-468.
  • Nygard, O., Refsum, H., Ueland, P.M. & Vollset, S.E. (1998) Major lifestyle determinants of plasma total homocysteine distribution: The hordaland homocysteine study. Am. J. Clin. Nutr. 67, 263-270.
  • Olivieri, O., Stanzial, A., Girelli, D., Trevisan, M.D., Guarini, P., Terzi, M., Caffi, S., Fontana, F., Casaril, M., Ferrari, S. & Corrocher, R. (1994) Selenium status, fatty acids, vitamin A and E, and aging: The Nove study. Am. J. Clin. Nutr. 60, 510-517.
  • Parnetti, L., Bottiglieri, T. & Lowenthal, D. (1997) Role of homocysteine in age-related vascular and non-vascular diseases. Aging (Milano) 9, 241-257.
  • Paolisso, G., Tagliamonte, M.R, Rizzo, M.R, Manzella, D., Gambardella, A., Varicchio, M. (1998) Oxidative stress and advancing age: Results in healthy centenarians J. Am. Geriatr. Soc. 46, 833-838.
  • Papanastasiou, P., Grass, L., Rodela, H., Patrikarea, A., Oreopoulos, D.G. & Diamandis, E. (1994) Immunological quantification of advanced glycosylation end-products in the serum of patients on hemodialysis or CAPD. Kidney Int. 46, 216-222.
  • Prencipe, L., Fossati, P. & Vanetti, G. (1978) Enzymatic determination of uric aid in serum with the trinder reaction. Quad. Sclavo. Diagn. 15, 382-394.
  • Rice-Evans, C.A., Diplock, A.T. & Symons, M.C.R. (1991) Techniques in Free Radical Research, pp. 47-50, Elsevier, Amsterdam.
  • Roberts, W.C. (1998) The heart at necropsy in centenarians. Am. J. Cardiol. 81, 1224-1225.
  • Rodriguez-Martinez, M.A. & Ruiz-Torres, A. (1992) Homeostasis between lipid peroxidation and antioxidant enzyme activities in healthy human aging. Mech. Ageing Dev. 66, 213-222.
  • Rodriguez, M.L., Smart, D., McCusker, C.A., Lamont, J.V., FitzGerald, S.P., Temml, C. & Lapin, A. (1996) Total antioxidant status (TAS) and glutathione reductase (GR) in geriatric patients. VIII Biennal Meeting for the International Society for Free Radical Research, Spain.
  • Sagara, Y., Dargusch, R., Chambers, D., Davis, J., Schubert, D. & Maher, P. (1998) Cellular mechanisms of resistance to chronic oxidative stress. Free Radical Biol. Med. 24, 1375-1389.
  • Sobczak, A., Skop, B. & Kula, B. (1999) Simultaneous determination of serum retinol and alpha- and gamma-tocopherol levels in type II diabetic patients using high-performance liquid chromatography with fluorescence detection. J. Chromatog. B. Biomed. Sci. Appl. 730, 265-271.
  • Sohal, R.S. & Orr, W.C. (1992) Relationship between antioxidants, prooxidants, and the aging process. Ann. N. Y. Acad. Sci. 663, 74-84.
  • Toh, S.Y., Thompson, G.W. & Basu, T.K. (1994) Riboflavin status of the elderly: Dietary intake and FAD-stimulationg effect on erythrocyte glutathione reductase coefficients. Eur. J. Clin. Nutr. 48, 654-659.
  • Vatassery, G.T., Krezowski, A.M. & Eckfeldt, J.H. (1983a) Vitamin E concentrations in human blood plasma and platelets. Am. J. Clin. Nutr. 37, 1020-1024.
  • Vatassery, G.T., Johnson, G.J. & Krezowski, A.M. (1983b) Changes in vitamin E concentrations in human plasma and platelets with age. J. Am. Coll. Nutr. 2, 369-375.
  • Warner, H.R. (1994) Superoxide dismutase, aging, and degenerative disease. Free Radical Biol. Med. 17, 249-258.
  • Wollesen, F., Brattstrom, L., Refsum, H., Ueland, P.M., Berglund, L. & Berbe, C. (1999) Plasma total homocysteine and cysteine in relation to glomerular filtration rate in diabetes mellitus. Kidney Int. 55, 1028-1035.
  • Yan, H. & Harding, J.J. (1997) Glycation-induced inactivation and loss of antigenicity of catalase and superoxide dismutase. Biochem. J. 328, 599-605.
  • Young, P.B., Molloy, A.M., Scott, J.M. & Kennedy, D.G. (1994) A rapid high performance liquid chromatographic method for determination of homocysteine in porcine tissue. J. Liquid Chromat. 17, 3553-3561.
  • Yu, B.P. (1996) Aging and oxidative stress: Modulation by dietary restriction. Free Radical Biol. Med. 21, 651-668.
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.