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2000 | 47 | 1 | 209-214
Article title

Cloning, expression, and crystallization of Cpn60 proteins from Thermococcus litoralis.

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EN
Abstracts
EN
Two genes of the extreme thermophilic archaeon Thermococcus litoralis homologous to those that code for Cpn60 chaperonins were cloned and expressed in Escherichia coli. Each of the Cpn60 subunits as well as the entire Cpn60 complex crystallize in a variety of morphological forms. The best crystals diffract to 3.6 Å resolution at room temperature and belong to the space group I422 with unit cell parameters a = b = 193.5 Å, c = 204.2 Å.
Publisher

Year
Volume
47
Issue
1
Pages
209-214
Physical description
Dates
published
2000
received
1999-10-6
accepted
2000-01-27
Contributors
author
  • Argonne National Laboratory, Biosciences Division, 9700 S. Cass Ave., Argonne, IL 60439, U.S.A.
  • Argonne National Laboratory, Biosciences Division, 9700 S. Cass Ave., Argonne, IL 60439, U.S.A.
author
  • University of Georgia, Department of Biochemistry & Molecular Biology, Athens, GA 30602, U.S.A
  • University of Georgia, Department of Biochemistry & Molecular Biology, Athens, GA 30602, U.S.A
  • Argonne National Laboratory, Biosciences Division, 9700 S. Cass Ave., Argonne, IL 60439, U.S.A.
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv47i1p209kz
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