Cloning, expression, and crystallization of Cpn60 proteins from Thermococcus litoralis.

• Authors: Jerzy Osipiuk , Mahalingam Sriram , Xuhong Mai , Michael W.W. Adams , Andrzej Joachimiak
• Languages of publication: EN

Abstracts

EN

Two genes of the extreme thermophilic archaeon Thermococcus litoralis homologous to those that code for Cpn60 chaperonins were cloned and expressed in Escherichia coli. Each of the Cpn60 subunits as well as the entire Cpn60 complex crystallize in a variety of morphological forms. The best crystals diffract to 3.6 Å resolution at room temperature and belong to the space group I422 with unit cell parameters a = b = 193.5 Å, c = 204.2 Å.

Keywords

EN

archaea; chaperonin; Thermococcus litoralis; heat-shock protein

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2000
• Volume: 47
• Issue: 1
• Pages: 209-214

Dates

published

2000

received

1999-10-6

accepted

2000-01-27

Contributors

author

Jerzy Osipiuk

• Argonne National Laboratory, Biosciences Division, 9700 S. Cass Ave., Argonne, IL 60439, U.S.A.

author

Mahalingam Sriram

• Argonne National Laboratory, Biosciences Division, 9700 S. Cass Ave., Argonne, IL 60439, U.S.A.

author

Xuhong Mai

• University of Georgia, Department of Biochemistry & Molecular Biology, Athens, GA 30602, U.S.A

author

Michael W.W. Adams

• University of Georgia, Department of Biochemistry & Molecular Biology, Athens, GA 30602, U.S.A

author

Andrzej Joachimiak

• Argonne National Laboratory, Biosciences Division, 9700 S. Cass Ave., Argonne, IL 60439, U.S.A.

References

• 1. Trent, J., Nimmesgern, E., Wall, J.S., Hartl, F.-U. & Horwich, A.L. (1991) Nature 354, 490-493.
• 2. Phipps, B.M., Typke, D., Hegerl, R., Volker, S., Hoffmann, A., Stetter, K.O. & Baumeister, W. (1993) Nature 361, 475-477.
• 3. Knapp, S., Schmidt-Krey, I., Hebert, H., Bergman, T., Jornvall, H. & Ladenstein, R. (1994) J. Mol. Biol. 242, 397-407.
• 4. Bult, C.J. et al. (1996) Science 273, 1058- 1073.
• 5. Waldmann, T., Nimmesgern, E., Nitsch, M., Peters, J., Pfeifer, G., Muller, S., Kellermann, J., Engel, A., Hartl, F.-U. & Baumeister, W. (1995) Eur. J. Biochem. 227, 848-856.
• 6. Archibald, J.M., Logsdon, J.M. & Doolittle, W.F. (1999) Curr. Biol. 9, 1053-1056.
• 7. Kagawa, H.K., Osipiuk, J., Maltsev, N., Overbeek, R., Quaite-Randall, E., Joachimiak, A. & Trent, J.D. (1995) J. Mol. Biol. 253, 712-725.
• 8. Quaite-Randall, E., Trent, J.D., Josephs, R. & Joachimiak, A. (1995) J. Biol. Chem. 270, 28818-28824.
• 9. Trent, J.D., Osipiuk, J. & Pinkau, T. (1990) J. Bacteriol. 172, 1478-1482.
• 10. Tian, G., Vainberg, I.E., Tap, W.D., Lewis, S.A. & Cowan, N.J. (1995) Nature 375, 250-253.
• 11. Guagliardi, A., Cerchia, L., Bartolucci, S. & Rossi, M. (1994) Protein Sci. 3, 1436-1443.
• 12. Ditzel, L., Lowe, J., Stock, D., Stetter, K.-O., Huber, H., Huber, R. & Steinbacher, S. (1998) Cell 93, 125-138.
• 13. Neuner, A., Jannasch, H.W., Belkin, S. & Stetter, K.O. (1990) Arch. Microbiol. 153, 205-207.
• 14. Reiter, W.-D., Palm, P. & Zillig, W. (1988) Nucleic Acids Res. 16, 1-19.
• 15. Reiter, W.-D., Palm, P. & Zillig, W. (1988) Nucleic Acids Res. 16, 2445-2459.
• 16. Freeman, B.C., Myers, M.P., Schumacher, R. & Morimoto, R. (1995) EMBO J. 14, 2281-2292.
• 17. Hemmingsen, S.M., Woolfor, D.C., Van Der Vies, S.M., Tilly, K., Dennis, D.T., Georgopoulos, C.P., Hendrix, R.W. & Ellis, R.J. (1988) Nature 333, 330-334.
• 18. Burnett, P.P., Dorwich, A.L. & Low, K.B. (1994) J. Bacteriol. 176, 6980-6985.
• 19. Kawarabayasi, Y., et al. (1998) DNA Res. 5, 147-155.
• 20. Yoshida, T., Yohda, M., Iida, T., Maruyama, T., Taguchi, H., Yazaki, K., Ohta, T., Odaka, M., Endo, I. & Kagawa, Y. (1997) J. Mol. Biol. 273, 635-645.
• 21. Osipiuk, J. & Joachimiak, A. (1997) Biochem. Biophys. Acta 1353, 253-265.
• 22. Otwinowski, Z. & Minor, W. (1995) The HKL Manual, Yale University Press, New Haven, CT.
• 23. Nitsch, M., Klumpp, M., Lupas, A. & Baumeister, W. (1997) J. Mol. Biol. 267, 142-149.
• 24. Brünger, A.T., Adams, P.D., Clore, G.M., DeLano, W.L., Gros, P., Grosse-Kunstleve, R. W., Jiang, J.S., Kuszewski, J., Nilges, M., Pannu, N.S., Read, R.J., Rice, L.M., Simonson, T. & Warren, G.L. (1998) Acta Cryst. D 54, 905-921.