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2000 | 47 | 1 | 165-171
Article title

Cytostatic and cytotoxic effects of (E)-2'-deoxy-2'-(fluoromethylene)-cytidine on a solid tumor and a leukemia cell line.

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Abstracts
EN
(E)-2'-deoxy-2'-(fluoromethylene)-cytidine (FMdC), a deoxycytidine analog displaying a very high toxicity toward a variety of solid tumor cell lines and xenografts, is activated intracellularly by deoxycytidine kinase (dCK). We have compared cytotoxicity of FMdC towards a human promyeolocytic leukemia line HL-60 and a human colorectal carcinoma line COLO-205. Despite dCK activity being by far the highest in cells of lymphoid origin, the effects of FMdC were detectable at the lowest drug concentration only in a solid tumor cell line, and at higher concentrations they were qualitatively similar in the two tumor lines (increased cell protein content, cell cycle block and apoptosis). Apparently, low dCK activity in solid tumor cells sufficiently activates FMdC to yield cytotoxic effects, while high dCK activity in leukemia cells does not increase its cytotoxicity.
Publisher

Year
Volume
47
Issue
1
Pages
165-171
Physical description
Dates
published
2000
received
1999-10-25
Contributors
author
  • Laboratory of Experimental Pharmacology, Medical Research Center, Polish Academy of Sciences, Warszawa, Poland
  • Flow Cytometry Laboratory, Drug Institute, Warszawa, Poland
  • Flow Cytometry Laboratory, Drug Institute, Warszawa, Poland
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv47i1p165kz
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