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2000 | 47 | 1 | 103-112
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Effect of ethanol on structural transitions of DNA and polyphosphates under Ca2+ ions action in mixed solutions.

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In the present work using the IR spectroscopy method the effect of ethanol on structural transitions of DNA and polyphosphates under the action of Ca2+ ions in mixed solutions containing ethanol (0-25 vol.%) was studied. It was shown that, on its interaction with Ca2+ ions, in aqueous and mixed solutions DNA becomes transformed into compact form. With the increase of concentration of ethanol the degree of Ca2+-induced DNA compactisation rises. It was found that, in mixed solutions containing ethanol, Ca2+-induced DNA compactisation depends not only on the solution's dielectric permeability but also on the solution structure. On stabilisation of the water structure in the presence of low ethanol concentrations a stabilisation of the DNA macromolecule occurs that leads to the increase of the Ca2+ ion concentration necessary for DNA compactisation. Comparison of the effects of ethanol on Ca2+-induced structural transitions in DNA and polyphosphates in mixed solvents permits to suppose that at alcohol concentrations in solution resulting in disruption of the water spatial structure, some peculiarities are observed in the behavior of those molecules whose hydrophobic interactions are essential.

Physical description
  • Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine, 47 Lenin Ave., 310164 Kharkov, Ukraine
  • Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine, 47 Lenin Ave., 310164 Kharkov, Ukraine
  • 1. Pearson, N.D. & Prescott, C.D. (1997) RNA as drug target. Chemistry & Biology 4, 409-414.
  • 2. Gao, Y.G., Liaw, Y.C., Robinson, H. & Wang, A.H. (1990) Binding of the antitumor drug nogalamycin and its derivatives to DNA: Structural comparison. Biochemistry 29, 10307-10316.
  • 3. Duguid, J., Bloomfield, V.A., Benevides, J. & Thomas, G.J., Jr. (1993) Raman spectroscopy of DNA-metal complexes. I. Interactions and conformational effects of the divalent cations: Mg, Ca, Sr, Ba, Mn, Co, Ni, Cu, Pd, and Cd. Biophys. J. 65, 1916-1928.
  • 4. Langlais, M., Tajmir-Riahi, H.A. & Savoie, R. (1990) Raman spectroscopic study of the effects of Ca2+, Mg2+, Zn2+, and Cd2+ ions on calf thymus DNA: Binding sites and conformational changes. Biopolymers 30, 743-752.
  • 5. Braunlin, W.H., Drakenberg, T. & Nordenskiold, L. (1992) Ca2+ binding environments on natural and synthetic polymeric DNA's. J. Biomol. Struct. Dyn. 10, 333-343.
  • 6. Xu, Y.C. & Bremer, H. (1997) Winding of the DNA helix by divalent metal ions. Nucleic Acids Res. 25, 4067-4071.
  • 7. Blagoi, Yu.P., Sorokin, V.A. & Valeev, V.A. (1980) Spectral studies of binding between DNA bases and magnesium or calcium ions. Mol. Biol. 14, 595-605.
  • 8. Kornilova, S.V., Sorokin, V.A., Blagoi, Yu.P. et al. (1991) Interaction of DNA with divalent metal ions. Mol. Biol. 25, 648-657.
  • 9. Kornilova, S.V., Kapinos, L.E. & Blagoi, Yu.P. (1995) Interaction of DNA with calcium ions by a vibrational spectroscopy. Mol. Biol. 29, 574-584.
  • 10. Blagoi, Yu.P., Galkin, V.L., Gladchenko, G.O., Kornilova, S.V., Sorokin, V.A. & Shkorbatov, A.G. (1991) Metal complexes of Nucleic Acids in Solutions; pp. 1-272, Naukova Dumka, Kiev (in Russian).
  • 11. Duguid, J.G. & Bloomfield, V.A. (1995) Aggregation of melted DNA by divalent metal ion-mediated cross-linking. Biophys. J. 69, 2642-2648.
  • 12. Blagoi, Yu.P., Sorokin, V.A., Valeev, V.A. & Gladchenko, G.O. (1983) Effects of calcium and manganese ions on the helix-coil transition of DNA. Biopolymers 22, 1641-1656.
  • 13. Kornilova, S.V., Iasem, P., Grigor'ev, D.N. et al. (1997) Calorimetric study of Ca2+ and Mn2+ ions' effect on DNA helix-coil transition. Biofizika 42, 599-606.
  • 14. Andrushchenko, V.V., Kornilova, S.V., Hackl, E.V. et al. (1997) IR-Spectroscopic studies of divalent metal ion effects on DNA hydration. J. Mol. Struct. 408/409, 225-228.
  • 15. Hackl, E.V., Kornilova, S.V., Kapinos, L.E. et al. (1997) Study of Ca2+, Mn2+, Cu2+ ion binding to DNA in solutions by means of IR-spectroscopy. J. Mol. Struct. 408/409, 229-232.
  • 16. Kornilova, S., Hackl, E., Kapinos, L., Andruszenko, V. & Blagoi, Yu.P. (1998) DNA interaction with biologicaly active metal ions. Cooperativity of metal ion binding at DNA compactization. Acta Biochim. Polon. 45, 107-117.
  • 17. Hackl, E.V., Kornilova, S.V. & Blagoi, Yu.P. (1998) DNA compactisation in Cu2+ ions presence in aqueous and aqueous-alcohol solutions. Visnyk Problem Biologii i Mediciny 8, 41-51 (in Russian).
  • 18. Bloomfield, V.A. (1998) DNA condensation by multivalent cations. Biopolymers 44, 269-282.
  • 19. Bloomfield, V.A. (1996) DNA condensation. Curr. Opin. Struct. Biol. 6, 334-341.
  • 20. Arscott, P.G., Ma, Ch., Wenner, J. & Bloomfield, V.A. (1995) DNA condensation by cobalt-hexaamine (III) in alcohol-water mixtures: Dielectric constant and other solvent effects. Biopolymers 36, 345-364.
  • 21. Wilson, R.W. & Bloomfield, V.A. (1979) Counterion-induced condensation of deoxyribonucleic acid. A light-scattering study. Biochemistry 18, 2192-2196.
  • 22. Votavova, H., Kucerova, D., Felsberg, J. & Sponar, J. (1986) Changes in conformation, stability and condensation of DNA by univalent and divalent cations in methanol-water mixtures. J. Biomol. Struct. Dyn. 4, 477-489.
  • 23. Lando, D.Iu., Egorova, V.P. & Krot, V.I. (1996) Determination of the level of protein contaminants in high-purity DNA preparations. Mol. Biol. 30, 701-706.
  • 24. Taillandier, E. & Liquier, J. (1992) Infrared spectroscopy of DNA. Methods Enzymol. 211, 307-360.
  • 25. Manning, G.S. (1978) The molecular theory of polyelectrolyte solutions with applications to the electrostatic properties of polynucleotides. Q. Rev. Biophys. 11, 179-246.
  • 26. Krestov, G.A. (1973) Thermodynamics of ionic processes in solutions. Chimia, pp. 1-272, Leningrad (in Russian).
  • 27. Arnett, E.M., Kover, W.B. & Larter, J.V. (1969) Heat capacities of organic compounds in solution. I. Low-molecular-weight alcohols in water. J. Am. Chem. Soc. 91, 4028-4034.
  • 28. Frisman, E.V., Veselkov, A.N., Slonitsky, S.V. & Karavaev, L.S. (1974) The influence of alcohol-water solvents on the conformation of deoxyribonucleic acid. Biopolymers 13, 2169- 2178.
  • 29. Veselkov, A.N. & Frisman, E.V. (1979) Conformation of the DNA molecule in alcohol-water solvents. Mol. Biol. 13, 184-191 (in Russian).
  • 30. Tajmir-Riahi, H.A., Ahmad, R., Naoui, M. & Diamantoglou, S. (1995) The effect of HCl on the solution structure of calf thymus DNA: A comparative study of DNA denaturation by proton and metal cations using Fourier transform IR difference spectroscopy. Biopolymers 35, 493-501.
  • 31. Nedjar-Arroume, N., Castellano, A. & Piot, J.M. (1993) Stabilizing effect of water/alcohol solvents towards autoxidation of human hemoglobin. Biotech. Appl. Biochem. 18, 25-35.
  • 32. Lozano, P., Combes, D. & Iborra, J.L. (1994) Effect of polyols on alpha-chymotrypsin thermostability: A mechanistic analysis of the enzyme stabilization. J. Biotechnol. 35, 9-18.
  • 33. Sanyude, S., Locock, R.A. & Pagliaro, L.A. (1991) Stability of aspartame in water: Organic solvent mixtures with different dielectric constants. J. Pharm. Sci. 80, 674-676.
  • 34. Xie, G. & Timasheff, S.N. (1997) Mechanism of the stabilization of ribonuclease A by sorbitol: Preferential hydration is greater for the denatured then for the native protein. Protein Sci. 6, 211-221.
  • 35. Timasheff, S.N. (1995) Solvent stabilization of protein structure. Methods Mol. Biol. 40, 253-269.
  • 36. Timasheff, S.N. (1993) The control of protein stability and association by weak interactions with water: How do solvents affect these processes? Annu. Rev. Biophys. Biomol. Struct. 22, 67-97.
  • 37. Timasheff, S.N. (1992) Water as ligand: Preferential binding and exclusion of denaturants in protein unfolding. Biochemistry 31, 9857- 9864.
  • 38. Arakawa, T. & Timasheff, S.N. (1985) The stabilization of proteins by osmolytes. Biophys. J. 47, 411-414.
  • 39. Frisman, E.V., Zarubina, O.P., Zyrianova, I.M. et. al. (1997) The role of ethanol during gamma-irradiation of water-salt DNA solutions. Biofizika 42, 117-124.
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