Inhibitory effect of resveratrol on free radical generation in blood platelets

• Authors: Beata Olas , Halina M. Żbikowska , Barbara Wachowicz , Tadeusz Krajewski , Andrzej Buczyński , Arleta Magnuszewska
• Languages of publication: EN

Abstracts

EN

Resveratrol (3,4',5-trihydroxystilbene), a compound found in many plants, has been shown to prevent coronary heart diseases and to exert a variety of antiinflammatory and anticancerogenic effects. It is effective in lowering the level of serum lipids and in inhibiting platelet aggregation. We evaluated the effect of trans-resveratrol on the production of free radicals in pig blood platelets and showed that resveratrol inhibited the production of different reactive oxygen species (O^·_2H^2O^2, singlet oxygen and organic radicals) measured by the luminol-dependent chemiluminescence in resting platelets (P < 0.05). Resveratrol inhibited also the generation of radicals in platelets activated by thrombin (P < 0.05). Treatment of platelets with resveratrol at concentrations of 6.25 and 12.5 μg/ml caused a statistically insignificant increase in the production of O^·-_2 in these cells, as measured by reduction of cytochrome c; however, at higher doses (25, 50 and 100 μg/ml) resveratrol distinctly reduced the generation of O^·-_2 in platelets (P < 0.05). We suggest that free radicals play an important role in the reduced reactivity of blood platelets induced by resveratrol.

Keywords

EN

resveratrol; blood platelet; free radicals

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 1999
• Volume: 46
• Issue: 4
• Pages: 961-966

Dates

published

1999

received

1999-05-26

revised

1999-07-13

accepted

1999-11-23

Contributors

author

Beata Olas

• Department of General Biochemistry, Institute of Biochemistry, University of Łódź, St. Banacha 12/16, 90-237 Łódź, Poland

author

Halina M. Żbikowska

• Department of General Biochemistry, Institute of Biochemistry, University of Łódź, St. Banacha 12/16, 90-237 Łódź, Poland

author

Barbara Wachowicz

• Department of General Biochemistry, Institute of Biochemistry, University of Łódź, St. Banacha 12/16, 90-237 Łódź, Poland

author

Tadeusz Krajewski

• Department of General Biochemistry, Institute of Biochemistry, University of Łódź, St. Banacha 12/16, 90-237 Łódź, Poland

author

Andrzej Buczyński

• Department of Preventive Medicine, Military School of Medicine, Łódź, Poland

author

Arleta Magnuszewska

• Department of General Biochemistry, Institute of Biochemistry, University of Łódź, St. Banacha 12/16, 90-237 Łódź, Poland