Identification of actin from hepatoma Morris 5123 cells

• Authors: Dorota Nowak , Agata Kochman , Maria Malicka-Błaszkiewicz
• Languages of publication: EN

Abstracts

EN

The hepatoma Morris 5123 tumor growth is accompanied by changes in actin content and polymerization (Malicka-Błaszkiewicz et al. (1995) Mat. Med. Pol., 27, 115-118;  Nowak et al. (1995) J. Exp. Cancer Res. 14, 37-40). Presently actin isoforms from cytosol and  cytoskeleton fractions were separated by SDS/PAGE and identified with antibodies directed against different actin isoforms. Actin isolated from the cytosol by affinity chromatography on DNase I bound to agarose shows the presence of only one protein spot on 2D gel electrophoresis corresponding to the mobility of the rabbit α skeletal muscle actin (M^r 43000) and isoelectric point equal to 5.3. It interacts only with monoclonal anti β actin isoform antibodies, posing the question of differential affinity of actin isoforms to DNase I.

Keywords

EN

actin isoforms; affinity chromatography; hepatoma Morris 5123; actin

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 1999
• Volume: 46
• Issue: 4
• Pages: 949-959

Dates

published

1999

received

1989-11-24

revised

1999-05-18

accepted

1999-11-20

Contributors

author

Dorota Nowak

• Department of Cell Pathology, Institute of Biochemistry and Molecular Biology, University of Wrocław, S. Przybyszewskiego 63, 51-148 Wrocław, Poland

author

Agata Kochman

• Department of Pathological Anatomy, Medical Academy of Wrocław, T. Chałubińskiego 10, Wrocław, Poland

author

Maria Malicka-Błaszkiewicz

• Department of Cell Pathology, Institute of Biochemistry and Molecular Biology, University of Wrocław, S. Przybyszewskiego 63, 51-148 Wrocław, Poland