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1999 | 46 | 4 | 949-959
Article title

Identification of actin from hepatoma Morris 5123 cells

Content
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Languages of publication
EN
Abstracts
EN
The hepatoma Morris 5123 tumor growth is accompanied by changes in actin content and polymerization (Malicka-Błaszkiewicz et al. (1995) Mat. Med. Pol., 27, 115-118;  Nowak et al. (1995) J. Exp. Cancer Res. 14, 37-40). Presently actin isoforms from cytosol and  cytoskeleton fractions were separated by SDS/PAGE and identified with antibodies directed against different actin isoforms. Actin isolated from the cytosol by affinity chromatography on DNase I bound to agarose shows the presence of only one protein spot on 2D gel electrophoresis corresponding to the mobility of the rabbit α skeletal muscle actin (M^r 43000) and isoelectric point equal to 5.3. It interacts only with monoclonal anti β actin isoform antibodies, posing the question of differential affinity of actin isoforms to DNase I.
Publisher

Year
Volume
46
Issue
4
Pages
949-959
Physical description
Dates
published
1999
received
1989-11-24
revised
1999-05-18
accepted
1999-11-20
Contributors
author
  • Department of Cell Pathology, Institute of Biochemistry and Molecular Biology, University of Wrocław, S. Przybyszewskiego 63, 51-148 Wrocław, Poland
author
  • Department of Pathological Anatomy, Medical Academy of Wrocław, T. Chałubińskiego 10, Wrocław, Poland
  • Department of Cell Pathology, Institute of Biochemistry and Molecular Biology, University of Wrocław, S. Przybyszewskiego 63, 51-148 Wrocław, Poland
References
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv46i4p949kz
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