Heparin- and Zn^2+-binding proteins from boar seminal plasma

• Authors: Dariusz Hołody , Jerzy Strzeżek
• Languages of publication: EN

Abstracts

EN

Low molecular mass, heparin-binding proteins from seminal plasma play an important role in gametes interaction whereas plasmatic Zn^{2+}-binding proteins stabilize chromatin and plasmalemma structures and protect spermatozoa in the female reproductive tract. By means of affinity chromatography the heparin- and Zn^{2+}-binding proteins were isolated from boar seminal plasma and both preparations were analyzed by reverse HPLC. Most of the proteins bound to heparine and Zn^{2+}-ions were classified as spermadhesins. Three fractions binding exclusively Zn^{2+} were isolated. They differ in amino-acid composition, content of glucosamine and content of protein components revealed by SDS/PAGE.

Keywords

EN

boar seminal plasma; heparin-binding proteins; Zn^{2+}-binding proteins

• Journal: Acta Biochimica Polonica
• Year: 1999
• Volume: 46
• Issue: 4
• Pages: 935-939

Dates

published

1999

received

1999-02-11

revised

1999-07-07

accepted

1999-10-21

Contributors

author

Dariusz Hołody

• Department of Animal Biochemistry, Olsztyn University of Agriculture and Technology, Oczapowski 5, 10-718 Olsztyn-Kortowo, Poland

author

Jerzy Strzeżek

• Department of Animal Biochemistry, Olsztyn University of Agriculture and Technology, Oczapowski 5, 10-718 Olsztyn-Kortowo, Poland