Low molecular mass products of depolymerization of purified mucin - attempts at isolation and characterization

• Authors: Iwona Minkiewicz , Andrzej Gindzieński
• Languages of publication: EN

Abstracts

EN

Samples of crude mucin were incubated at room temperature for 48 and 96 h in a sodium azide containing buffer, pH 7.0. Then each sample was purified, reduced and alkylated with iodo[^14C]acetamide. Electrophoretic analysis demonstrated that radioactivity was incorporated into the mucin subunits and proteins of 100 and 140 kDa. The results of our experiments suggest that the released proteins can be a part of mucin molecule, cleaved by proteolysis and reduction of disulfide bridges.

Keywords

EN

disulfide bridges; subunits; isolation; mucin

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 1999
• Volume: 46
• Issue: 4
• Pages: 928-933

Dates

published

1999

received

1999-02-11

revised

1999-06-21

accepted

1999-10-10

Contributors

author

Iwona Minkiewicz

• Department of General and Organic Chemistry, Institute of Chemistry, Medical Academy of Białystok, A. Mickiewicza 2, 15-230 Białystok 8, Poland

author

Andrzej Gindzieński

• Department of General and Organic Chemistry, Institute of Chemistry, Medical Academy of Białystok, A. Mickiewicza 2, 15-230 Białystok 8, Poland