SDS/PAGE characteristics of protein kinases tightly associated with chick embryo brain ribosomes

• Authors: Maria Sanecka-Obacz
• Languages of publication: EN

Abstracts

EN

Protein kinases tightly associated with chick embryo brain ribosomes washed with Triton X-100 and KCl were characterized by their ability to phosphorylate ribosomes and two exogenous substrates, histone IIA and casein. c-AMP-dependent kinase (PKA) and casein kinases (CK1, CK2) were examined in the presence of specific modulators by SDS/ PAGE followed by  renaturation in gel assay according to Kameshita & Fujisawa (Anal. Biochem. 1989, 183, 139-143). Basing on these data it can be presumed that PKA activity increases, but the levels of CK2 and CK1 decrease during chick embryo development.

Keywords

EN

protein phosphorylation; chick brain development; ribosomal kinases; CK2; PKA; CK1; fetal brain; brain ribosomes

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 1999
• Volume: 46
• Issue: 4
• Pages: 911-917

Dates

published

1999

received

1989-11-19

revised

1999-06-21

accepted

1999-10-05

Contributors

author

Maria Sanecka-Obacz

• Department of Physiological Chemistry, Medical Academy, 20-123 Lublin, Lubartowska 85, Poland