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1999 | 46 | 4 | 901-910
Article title

Extraribosomal function of the acidic ribosomal P1-protein YP1α from Saccharomyces cerevisiae

Content
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EN
Abstracts
EN
The yeast acidic ribosomal P-proteins YP1α, YP1β, YP2a and YP2b were studied for a possible transactivation potential beside their ribosomal function. The fusions of P-proteins with the GAL4 DNA-binding domain were assayed toward their transcriptional activity with the aid of reporter genes in yeast. Two of the P-proteins, YP1α and YP1β, exhibited transactivation potential, however, only YP1α can be regarded as a potent transactivator. This protein was able to transactivate a reporter gene associated with two distinct promoter systems, GAL1 or CYC1. Additionally, truncated proteins of YP1α and YP1β were analyzed. The N-terminal part of YP1α fused to GAL4-BD showed transactivation potential but the C-terminal part did not. Our results suggest a putative extraribosomal function for these ribosomal proteins which consequently may be classified as "moonlighting" proteins.
Publisher

Year
Volume
46
Issue
4
Pages
901-910
Physical description
Dates
published
1999
received
1999-07-05
accepted
1999-11-04
Contributors
  • Maria Curie-Skłodowska University, Institute of Microbiology and Biotechnology, Department of Molecular Biology, Akademicka 19, 20-033 Lublin, Poland
  • Odense University, Institute of Biochemistry, Campusvej 55, DK-5230 Odense, Denmark
  • Maria Curie-Skłodowska University, Institute of Microbiology and Biotechnology, Department of Molecular Biology, Akademicka 19, 20-033 Lublin, Poland
References
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv46i4p901kz
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